Characterization of & induction of immune response to anti-idiotypic antibodies for JE virus.

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dc.contributor.authorGhanekar, S Aen_US
dc.contributor.authorCecilia, Den_US
dc.contributor.authorKutubuddin, Men_US
dc.contributor.authorGoverdhan, M Ken_US
dc.contributor.authorDandawate, C Nen_US
dc.contributor.authorBanerjee, Ken_US
dc.contributor.authorGhosh, S Nen_US
dc.date.accessioned1991-05-01en_US
dc.date.accessioned2009-05-27T08:15:00Z
dc.date.available1991-05-01en_US
dc.date.available2009-05-27T08:15:00Z
dc.date.issued1991-05-01en_US
dc.description.abstractAnti-idiotypic antibodies (anti-Ids, Ab2s) were prepared by immunizing rabbits with two murine monoclonal antibodies (Ab1) having specificities for two independent haemagglutinin (HA) epitopes on JE virus [viz., Hs-1, monoclonal antibody (MAb) specific for Japanese encephalitis virus (JEV) and Hx-1, MAb common to flaviviruses]. Anti-Hs-1 (S-Ab2) and Anti-Hx-1 (X-Ab2) reacted specifically with the immunizing Ab1. In addition, they could react with other MAbs whose reactivity was similar to their immunizing homologous Ab1. The paratope inhibition assay indicated that both anti-idiotypes recognized paratope related idiotopes on their respective Ab1 and could therefore be designated as Ab2 beta. Experimental animals (Swiss mice, Balb/c mice and guineapigs) immunized with S-Ab2 or X-Ab2 produced anti-JE virus antibodies (Ab3) which could be detected by enzyme linked immunosorbent assay, immunofluorescence, haemagglutination inhibition and neutralization tests. The anti-idiotypes were also found to stimulate a cellular immune response in vitro as assessed by 3H thymidine incorporation by lymphocytes from JE vaccinated individuals and experimentally immunized Balb/c mice. The findings of the present study suggest that both the anti-Id antibodies are homobodies which may act as surrogate antigens to manipulate the immune response against JEV.en_US
dc.description.affiliationNational Institute of Virology, Pune.en_US
dc.identifier.citationGhanekar SA, Cecilia D, Kutubuddin M, Goverdhan MK, Dandawate CN, Banerjee K, Ghosh SN. Characterization of & induction of immune response to anti-idiotypic antibodies for JE virus. Indian Journal of Medical Research. 1991 May; 93(): 131-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/23777
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Anti-Idiotypic --immunologyen_US
dc.subject.meshAntibodies, Monoclonal --immunologyen_US
dc.subject.meshAntibodies, Viral --biosynthesisen_US
dc.subject.meshEncephalitis Virus, Japanese --immunologyen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmune Sera --immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshRabbitsen_US
dc.titleCharacterization of & induction of immune response to anti-idiotypic antibodies for JE virus.en_US
dc.typeJournal Articleen_US
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