Fatty acid synthesis by isolated leucoplasts from developing Brassica seeds: role of glycolytic intermediates as the source of carbon and energy.

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dc.contributor.authorGupta, Ren_US
dc.contributor.authorSingh, Ren_US
dc.date.accessioned1996-12-01en_US
dc.date.accessioned2009-05-27T09:03:35Z
dc.date.available1996-12-01en_US
dc.date.available2009-05-27T09:03:35Z
dc.date.issued1996-12-01en_US
dc.description.abstractFatty acid synthesis from Na [1-14C] acetate in leucoplasts isolated from developing seeds of Brassica campestris was completely dependent on exogenous supply of ATP. None of the intermediates of glycolysis or pentose phosphate pathway tested could replace ATP in the reaction mixture. In absence of exogenously supplied ATP, maximum activity was obtained with glu-6-P (68%) followed by fru-6-P (50%) and PEP (44%), respectively. With other intermediates as energy sources, the activity ranged from 1 to 38%. In complementary experiments (presence of ATP), none of the metabolites gave activity higher than the ATP control activity. Under optimum conditions for fatty acid synthesis from acetate, Brassica leucoplasts readily utilized labelled glucose as the substrate for fatty acid synthesis. Omission of NADH and NADPH individually from the reaction mixtures containing labelled glucose resulted only in 46 and 20% loss in activity, respectively, compared to the corresponding losses of 56 and 50%, when labelled acetate was used as the substrate. Similarly, deletion of ATP from the reaction mixture containing glucose as the substrate decreased the rate of fatty acid synthesis by about 65%, while the corresponding decrease with acetate as the substrate was 96%. Inclusion of 5 mM cold acetate, pyruvate, malate and glu-6-P in the reaction mixture containing glucose as the labelled substrate reduced label incorporation into fatty acids by 38 to 69%, maximum reduction being observed with pyruvate followed by glu-6-P, acetate and malate, respectively. With labelled acetate as the substrate, maximum reduction in label incorporation was obtained with cold glucose (5 mM) followed by glu-6-P, pyruvate and malate, respectively. The study demonstrated the operation of complete glycolytic pathway in Brassica leucoplasts, allowing the plastids to use glucose as a source of carbon, reducing power and energy for fatty acid synthesis.en_US
dc.description.affiliationDepartment of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar, India.en_US
dc.identifier.citationGupta R, Singh R. Fatty acid synthesis by isolated leucoplasts from developing Brassica seeds: role of glycolytic intermediates as the source of carbon and energy. Indian Journal of Biochemistry & Biophysics. 1996 Dec; 33(6): 478-83en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26315
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAcetates --metabolismen_US
dc.subject.meshAdenosine Triphosphate --metabolismen_US
dc.subject.meshBrassica --metabolismen_US
dc.subject.meshEnergy Metabolismen_US
dc.subject.meshFatty Acids --biosynthesisen_US
dc.subject.meshGlucose --metabolismen_US
dc.subject.meshGlycolysisen_US
dc.subject.meshMonosaccharides --metabolismen_US
dc.subject.meshNAD --metabolismen_US
dc.subject.meshNADP --metabolismen_US
dc.subject.meshPentose Phosphate Pathwayen_US
dc.subject.meshPlastids --metabolismen_US
dc.subject.meshSeeds --metabolismen_US
dc.titleFatty acid synthesis by isolated leucoplasts from developing Brassica seeds: role of glycolytic intermediates as the source of carbon and energy.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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