Purification and properties of an extracellular pectin lyase produced by the strain of Penicillium oxalicum in solid-state fermentation.
dc.contributor.author | Yadav, Sangeeta | en_US |
dc.contributor.author | Shastri, N V | en_US |
dc.date.accessioned | 2007-08-01 | en_US |
dc.date.accessioned | 2009-05-27T09:48:13Z | |
dc.date.available | 2007-08-01 | en_US |
dc.date.available | 2009-05-27T09:48:13Z | |
dc.date.issued | 2007-08-01 | en_US |
dc.description.abstract | A pectin lyase (PNL, EC 4.2.2.10) produced extracellularly by the strain of Penicillium oxalicum in solid-state fermentation medium containing deoiled mandarin orange peel meal was purified to apparent homogeneity by a protocol that included ammonium sulfate precipitation, DEAE-Sephadex A-50 and Sephadex G-100 chromatography. The enzyme had molecular mass of 50 kD, as determined by SDS- PAGE and showed optimum pH and temperature at 8.0 and 50 degrees C respectively. It had an isoelectric point (pI) of 5.0 and showed a K(m) of 1.1 mg/ml of citrus pectin. The enzyme was strongly inhibited by Mo4+, Ag+ and Pb2+ and also by polyphenolic compounds, in particular tannic acid. | en_US |
dc.description.affiliation | Department of Biochemistry, RTM Nagpur University, Amravati Road, Nagpur 440 010, India. sangeeta_rahul@rediffmail.com | en_US |
dc.identifier.citation | Yadav S, Shastri NV. Purification and properties of an extracellular pectin lyase produced by the strain of Penicillium oxalicum in solid-state fermentation. Indian Journal of Biochemistry & Biophysics. 2007 Aug; 44(4): 247-51 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/28606 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.asp | en_US |
dc.subject.mesh | Extracellular Space --metabolism | en_US |
dc.subject.mesh | Fermentation | en_US |
dc.subject.mesh | Penicillium --enzymology | en_US |
dc.subject.mesh | Polysaccharide-Lyases --isolation & purification | en_US |
dc.title | Purification and properties of an extracellular pectin lyase produced by the strain of Penicillium oxalicum in solid-state fermentation. | en_US |
dc.type | Journal Article | en_US |
dc.type | Research Support, Non-U.S. Gov't | en_US |
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