Purification and properties of an extracellular pectin lyase produced by the strain of Penicillium oxalicum in solid-state fermentation.

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dc.contributor.authorYadav, Sangeetaen_US
dc.contributor.authorShastri, N Ven_US
dc.date.accessioned2007-08-01en_US
dc.date.accessioned2009-05-27T09:48:13Z
dc.date.available2007-08-01en_US
dc.date.available2009-05-27T09:48:13Z
dc.date.issued2007-08-01en_US
dc.description.abstractA pectin lyase (PNL, EC 4.2.2.10) produced extracellularly by the strain of Penicillium oxalicum in solid-state fermentation medium containing deoiled mandarin orange peel meal was purified to apparent homogeneity by a protocol that included ammonium sulfate precipitation, DEAE-Sephadex A-50 and Sephadex G-100 chromatography. The enzyme had molecular mass of 50 kD, as determined by SDS- PAGE and showed optimum pH and temperature at 8.0 and 50 degrees C respectively. It had an isoelectric point (pI) of 5.0 and showed a K(m) of 1.1 mg/ml of citrus pectin. The enzyme was strongly inhibited by Mo4+, Ag+ and Pb2+ and also by polyphenolic compounds, in particular tannic acid.en_US
dc.description.affiliationDepartment of Biochemistry, RTM Nagpur University, Amravati Road, Nagpur 440 010, India. sangeeta_rahul@rediffmail.comen_US
dc.identifier.citationYadav S, Shastri NV. Purification and properties of an extracellular pectin lyase produced by the strain of Penicillium oxalicum in solid-state fermentation. Indian Journal of Biochemistry & Biophysics. 2007 Aug; 44(4): 247-51en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28606
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshExtracellular Space --metabolismen_US
dc.subject.meshFermentationen_US
dc.subject.meshPenicillium --enzymologyen_US
dc.subject.meshPolysaccharide-Lyases --isolation & purificationen_US
dc.titlePurification and properties of an extracellular pectin lyase produced by the strain of Penicillium oxalicum in solid-state fermentation.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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