RECK and TIMP-2 mediate inhibition of MMP-2 and MMP-9 by Annona muricata

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dc.contributor.authorDrishya, Gen_US
dc.contributor.authorNambiar, Jyotsnaen_US
dc.contributor.authorShaji, Sanu Ken_US
dc.contributor.authorVanuopadath, Muralidharanen_US
dc.contributor.authorAchuthan, Aen_US
dc.contributor.authorKumar, Abisheken_US
dc.contributor.authorAlias, Ashnaen_US
dc.contributor.authorSherif, Ayeshaen_US
dc.contributor.authorJoseph, Catherineen_US
dc.contributor.authorPdivyaen_US
dc.contributor.authorKumar, Damu Sunilen_US
dc.contributor.authorBose, Chinchuen_US
dc.contributor.authorNair, Sobha Vijayanen_US
dc.contributor.authorSsudarslalen_US
dc.contributor.authorKumar, Geetha Ben_US
dc.contributor.authorLakshmi, Sen_US
dc.contributor.authorNair, Bipin Gen_US
dc.date.accessioned2020-11-18T10:14:18Z
dc.date.available2020-11-18T10:14:18Z
dc.date.issued2020-07
dc.description.abstractUp-regulation of MMP-2 and MMP-9 plays a significant role in promoting cancer progression by degradingthe components of the extracellular matrix, thereby enhancing the migration of tumor cells. Although the antiproliferative and apoptotic effect of Annona muricata is well established, its effect on MMP-2 and MMP-9, amajor target in several types of cancers, has not been studied. Powdered samples of various parts of A.muricata like fruit, stem, seed, and twig extracted using aqueous methanol showed significant dose-dependentinhibition of MMP-2 and MMP-9 in a highly metastatic fibrosarcoma cell line, HT1080. Additionally, theseextracts also up-regulated the expression of several endogenous inhibitors of MMP-2 and MMP-9 likeREversion-inducing Cysteine-rich protein with Kazal motifs (RECK) and Tissue Inhibitor of Metalloproteinase-2 (TIMP-2). Furthermore, primary cells developed from tumor tissues obtained from patients notexposed to chemotherapy, also exhibited similar results. Remarkably, the inhibition of MMP-2 and MMP-9observed was tumor specific, with the A. muricata fruit extract showing only 2% inhibition in cells obtainedfrom normal tissues, when compared to 60% inhibition observed in cells obtained from tumor samples. Thepresent study elucidates a novel mechanism by which A. muricata extracts selectively exhibit their anti-canceractivity in tumor cells by down-regulating MMP-2 and MMP-9 that are important biomarkers in cancer.en_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.affiliationsLaboratory of Molecular Medicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram 695 011, Indiaen_US
dc.identifier.affiliationsAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Clappana P.O, Kollam 690 525, Indiaen_US
dc.identifier.citationDrishya G, Nambiar Jyotsna, Shaji Sanu K, Vanuopadath Muralidharan, Achuthan A, Kumar Abishek, Alias Ashna, Sherif Ayesha, Joseph Catherine, Pdivya, Kumar Damu Sunil, Bose Chinchu, Nair Sobha Vijayan, Ssudarslal, Kumar Geetha B, Lakshmi S, Nair Bipin G. RECK and TIMP-2 mediate inhibition of MMP-2 and MMP-9 by Annona muricata. Journal of Biosciences. 2020 Jul; : 1-11en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214269
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.volume45en_US
dc.source.urihttps://dx.doi.org//10.1007/s12038-020-00056-zen_US
dc.subjectAnnona muricataen_US
dc.subjectfibrosarcomaen_US
dc.subjectMMP-2 and MMP-9en_US
dc.subjectRECKen_US
dc.subjectTIMP-2en_US
dc.titleRECK and TIMP-2 mediate inhibition of MMP-2 and MMP-9 by Annona muricataen_US
dc.typeJournal Articleen_US
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