NMR-based comparative metabolomics of quiescent muscle cells

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dc.contributor.authorPUROHIT, GUNJANen_US
dc.contributor.authorRAMESH, AKILAen_US
dc.contributor.authorPATEL, ANANT Ben_US
dc.contributor.authorDHAWAN, JYOTSNAen_US
dc.date.accessioned2024-11-30T11:24:42Z
dc.date.available2024-11-30T11:24:42Z
dc.date.issued2024-05
dc.description.abstractAdult muscle tissue largely comprised of differentiated myofibers also harbors quiescent muscle-resident stem cells (MuSCs) that are responsible for its maintenance, repair and regeneration. Emerging evidence suggests that quiescent MuSCs exhibit a specific metabolic state, which is regulated during physiological and pathological alterations. However, a detailed understanding of the metabolic state of quiescent MuSCs and its alteration during activation and repair is lacking. Direct profiling of MuSCs in vivo is challenging because the cells are rare and dispersed, while isolation and enrichment leads to their activation and loss of quiescence. In this study, we employed 1H-nuclear magnetic resonance (NMR) spectroscopy to profile metabolites in an established culture model of quiescent MuSC-derived myoblasts and compared with activated, proliferative and differentiated muscle cells to determine the state-specific metabolome. We report that the proliferating and differentiated cells are highly enriched in metabolites involved in energy generation, the quiescent state is enriched in metabolites related to phospholipid catabolism (glycerophosphocholine and choline) and depleted for phosphocholine which is enriched in proliferating cells. We propose that the ratio of these metabolites may be useful as a biomarker of MuSC quiescence.en_US
dc.identifier.affiliationsCSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India; Present Address: Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, USAen_US
dc.identifier.affiliationsCSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, Indiaen_US
dc.identifier.affiliationsCSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, Indiaen_US
dc.identifier.affiliationsCSIR-Centre for Cellular and Molecular Biology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, Indiaen_US
dc.identifier.citationPUROHIT GUNJAN, RAMESH AKILA, PATEL ANANT B, DHAWAN JYOTSNA. NMR-based comparative metabolomics of quiescent muscle cells. Journal of Biosciences. 2024 May; 49: 1-14en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/237977
dc.languageenen_US
dc.publisherThe Indian Academy of Sciencesen_US
dc.relation.volume49en_US
dc.source.urihttps://doi.org/10.1007/s12038-024-00442-xen_US
dc.subjectBiomarkeren_US
dc.subjectG0en_US
dc.subjectmetabolic profileen_US
dc.subjectmyoblasten_US
dc.subjectnuclear magnetic resonance spectroscopyen_US
dc.subjectquiescenceen_US
dc.titleNMR-based comparative metabolomics of quiescent muscle cellsen_US
dc.typeJournal Articleen_US
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