How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India.
| dc.contributor.author | Harishankar, Anusha | |
| dc.contributor.author | Chandy, Mammen | |
| dc.contributor.author | Bhattacharya, Sanjay | |
| dc.date.accessioned | 2016-08-08T10:25:32Z | |
| dc.date.available | 2016-08-08T10:25:32Z | |
| dc.date.issued | 2015-10 | |
| dc.description.abstract | Development of a reliable, cost-effective cytomegalovirus quantitative polymerase chain reaction (QPCR) is a priority for developing countries. Manufactured kits are expensive, and availability can be inconsistent. Development of an in-house QPCR kit that is reliable and quality assured requires significant effort and initial investment. However, the rewards of such an enterprise are manifold and include an in-depth understanding of molecular reactions, and expertise in the development of further low-cost molecular kits. The experience of an oncology centre in Eastern India has been shared. Hopefully, this would provide a brief roadmap for such an initiative. Staff with adequate understanding of molecular processes are essential along with vital infrastructure for molecular research and development. | en_US |
| dc.identifier.citation | Harishankar Anusha, Chandy Mammen, Bhattacharya Sanjay. How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India. Indian Journal of Medical Microbiology. 2015 Oct-Dec; 33(4): 482-490. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/176501 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ijmm.org/article.asp?issn=0255-0857;year=2015;volume=33;issue=4;spage=482;epage=490;aulast=Harishankar | en_US |
| dc.subject | Cytomegalovirus | en_US |
| dc.subject | standardisation | en_US |
| dc.subject | troubleshooting | en_US |
| dc.subject | validation | en_US |
| dc.subject | viral load | en_US |
| dc.title | How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India. | en_US |
| dc.type | Article | en_US |