Productivity and biochemical properties of green tea in response to full-length and functional fragments of HpaG Xooc, a harpin protein from the bacterial rice leaf streak pathogen Xanthomonas oryzae pv. oryzicola.

Simple item page
dc.contributor.authorWu, Xiaojingen_US
dc.contributor.authorWu, Tingquanen_US
dc.contributor.authorLong, Juyingen_US
dc.contributor.authorYin, Qianen_US
dc.contributor.authorZhang, Yongen_US
dc.contributor.authorChen, Leien_US
dc.contributor.authorLiu, Ruoxueen_US
dc.contributor.authorGao, Tongchunen_US
dc.contributor.authorDong, Hansongen_US
dc.date.accessioned2007-09-24en_US
dc.date.accessioned2009-06-01T14:52:55Z
dc.date.available2007-09-24en_US
dc.date.available2009-06-01T14:52:55Z
dc.date.issued2007-09-24en_US
dc.description.abstractHarpin proteins from plant pathogenic bacteria can stimulate hypersensitive cell death (HCD), drought tolerance, defence responses against pathogens and insects in plants, as well as enhance plant growth. Recently, we identified nine functional fragments of HpaG;Xooc, a harpin protein from Xanthomonas oryzae pv.oryzicola, the pathogen that causes bacterial leaf streak in rice. Fragments HpaG;1-94'HpaG;10-42, and HpaG;62-138, which contain the HpaG;Xooc regions of the amino acid sequence as indicated by the number spans, exceed the parent protein in promoting growth, pathogen defence and HCD in plants. Here we report improved productivity and biochemical properties of green tea (Camellia sinensis) in response to the fragments tested in comparison with HpaG;Xooc and an inactive protein control. Field tests suggested that the four proteins markedly increased the growth and yield of green tea, and increased the leaf content of tea catechols, a group of compounds that have relevance in the prevention and treatment of human diseases. In particular, HpaG;1-94 was more active than HpaG;Xooc in expediting the growth of juvenile buds and leaves used as green tea material and increased the catechol content of processed teas. When tea shrubs were treated with HpaH;Xooc and HpaG;1-94 compared with a control, green tea yields were over 55% and 39% greater, and leaf catechols were increased by more than 64% and 72%, respectively. The expression of three homologues of the expansin genes, which regulate plant cell growth, and the CsCHS gene encoding a tea chalcone synthase, which critically regulates the biosynthesis of catechols, were induced in germinal leaves of tea plants following treatment with HpaG;1-94 or HpaG;Xooc. Higher levels of gene expression were induced by the application of HpaG;1-94 than HpaG;Xooc. Our results suggest that the harpin protein, especially the functional fragment HpaG;1-94, can be used to effectively increase the yield and improve the biochemical properties of green tea, a drink with medicinal properties.en_US
dc.description.affiliationKey Laboratory of Monitoring and Management of Plant Pathogens and Insect Pests, Ministry of Agriculture of China, Nanjing Agricultural University, Nanjing 210095, China.en_US
dc.identifier.citationWu X, Wu T, Long J, Yin Q, Zhang Y, Chen L, Liu R, Gao T, Dong H. Productivity and biochemical properties of green tea in response to full-length and functional fragments of HpaG Xooc, a harpin protein from the bacterial rice leaf streak pathogen Xanthomonas oryzae pv. oryzicola. Journal of Biosciences. 2007 Sep; 32(6): 1119-31en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111145
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshBacterial Proteins --chemistryen_US
dc.subject.meshCamellia sinensis --metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOryza sativa --microbiologyen_US
dc.subject.meshPeptide Fragments --chemistryen_US
dc.subject.meshPlant Diseases --microbiologyen_US
dc.subject.meshTea --chemistryen_US
dc.subject.meshXanthomonas --pathogenicityen_US
dc.titleProductivity and biochemical properties of green tea in response to full-length and functional fragments of HpaG Xooc, a harpin protein from the bacterial rice leaf streak pathogen Xanthomonas oryzae pv. oryzicola.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Journal of Biosciences