Study of Neuromuscular Transmission Under (i) Phenomenon of Fatigue, (ii) Site of Fatigue, (iii) Neuromuscular Blocking in an in-situ Rat Nerve Muscle Preparation: ANovel Approach to Nerve Muscle Physiology Experiment Teaching

dc.contributor.authorDas, Aranien_US
dc.contributor.authorChakraborty, Arpitaen_US
dc.contributor.authorSrivastav, Shivalen_US
dc.contributor.authorKaur, Simranen_US
dc.contributor.authorJain, Sumanen_US
dc.contributor.authorBade, Geetanjalien_US
dc.date.accessioned2020-04-23T07:40:18Z
dc.date.available2020-04-23T07:40:18Z
dc.date.issued2019-01
dc.description.abstractPurpose of the study: Nerve-muscle physiology is a very basic and vital module in undergraduate physiologycurriculum. The practical sessions on this system are demonstrated on amphibian (frog) nerve-musclepreparation, which becomes a limiting factor in most of the medical colleges of India. In this study, wepropose an alternative approach by using mammalian (rat) model for nerve-muscle physiology undergraduatepractical experiments.Materials and methods: Rat in-situ sciatic nerve-soleus muscle preparation was used to study neuromusculartransmission. Stimulation of nerve and recording of muscle contraction (force) were done by using digitalrecording system. To demonstrate fatigue, repetitive electrical stimulation was applied to nerve-musclepreparation and muscle twitches were recorded. A reduction in amplitude of contraction to 50% of their basalrecording was considered as onset of fatigue. To demonstrate site of fatigue, the muscle was stimulateddirectly after the fatigue was observed with nerve stimulation and muscle twitch recorded. To observe theeffect of neuromuscular blocking drug Pancuronium bromide, on neuromuscular transmission, in a separateset up the drug was injected in muscle belly at multiple sites and nerve was stimulated to elicit muscletwitch. The response (amplitude of muscle twitch) was compared with control (injection of 0.9% saline).Main findings: On repeated stimulation of nerve muscle preparation, initially there was an increase inamplitude of contraction but progressively amplitude went on decreasing. After development of fatigue ondirect muscle stimulation, amplitude recorded was same as the initial twitch amplitude. This demonstratesthat the site of fatigue is not the muscle. Further, as nerve is also non- fatigable, site of fatigue wasneuromuscular junction.Injection of Pancuronium bromide showed that the twitch amplitude decreased substantially than the control(saline injection) on stimulation of nerve, but on direct muscle stimulation amplitude of contraction remainedsame as initial, confirming the effect of drug on neuromuscular junction.en_US
dc.identifier.affiliationsDepartment of Physiology,AIIMS, New Delhien_US
dc.identifier.citationDas Arani, Chakraborty Arpita, Srivastav Shival, Kaur Simran, Jain Suman, Bade Geetanjali. Study of Neuromuscular Transmission Under (i) Phenomenon of Fatigue, (ii) Site of Fatigue, (iii) Neuromuscular Blocking in an in-situ Rat Nerve Muscle Preparation: ANovel Approach to Nerve Muscle Physiology Experiment Teaching. Indian Journal of Physiology and Pharmacology. 2019 Jan; 1: 79-85en_US
dc.identifier.issn0019-5499
dc.identifier.issn2582 – 2799
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/198921
dc.languageenen_US
dc.publisherScientific Scholaren_US
dc.relation.issuenumber1en_US
dc.relation.volume63en_US
dc.source.urihttps://ijpp.com/IJPP%20archives/2019_63_1/79-85.pdfen_US
dc.titleStudy of Neuromuscular Transmission Under (i) Phenomenon of Fatigue, (ii) Site of Fatigue, (iii) Neuromuscular Blocking in an in-situ Rat Nerve Muscle Preparation: ANovel Approach to Nerve Muscle Physiology Experiment Teachingen_US
dc.typeJournal Articleen_US
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