Major bovine brain stem glycoproteins sugar-specifically recognized by endogenous 14 kDa galactose-binding lectin.

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dc.contributor.authorKannan, V Men_US
dc.contributor.authorAppukuttan, P Sen_US
dc.date.accessioned1994-06-01en_US
dc.date.accessioned2009-05-27T09:56:02Z
dc.date.available1994-06-01en_US
dc.date.available2009-05-27T09:56:02Z
dc.date.issued1994-06-01en_US
dc.description.abstractIn order to identify the complementary glycoproteins (receptors) that are recognized in bovine brain stem by endogenous 14 kDa galactose-binding lectin (BBL), probable glycoproteins were first selected by affinity chromatography of soluble tissue glycoproteins on Rinicus communis agglutinin (RCA)-Sepharose since this lectin had similar sugar specificity to the endogenous lectin. From Western blot of RCA-binding glycoproteins, the lectin, as its peroxidase conjugate sugar-specifically recognized chiefly an 84 kDa glycoprotein subunit and a few minor subunits. On alkaline pH PAGE of the RCA-binding brain stem glycoproteins, a prominent fast moving protein was separated which, on electroelution and dot blotting, was also recognized by BBL sugar-specifically. This glycoprotein was composed of 55 kDa and 58 kDa subunits as seen by SDS-PAGE and was also immunologically distinct from the 84 kDa subunit. Qualitative test on dot blots of the electroeluted glycoproteins using peroxidase conjugates of plant lectins of varying specificities as well as the human serum anti-alpha-galactoside antibody indicated differences in carbohydrate composition between the 84 kDa subunit and the alkaline PAGE fast moving glycoprotein. Membrane-bound brain stem glycoproteins were not recognized by BBL.en_US
dc.description.affiliationNeurochemistry Division, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum, India.en_US
dc.identifier.citationKannan VM, Appukuttan PS. Major bovine brain stem glycoproteins sugar-specifically recognized by endogenous 14 kDa galactose-binding lectin. Indian Journal of Biochemistry & Biophysics. 1994 Jun; 31(3): 160-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28991
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain Stem --metabolismen_US
dc.subject.meshCarrier Proteins --metabolismen_US
dc.subject.meshCattleen_US
dc.subject.meshGalectinsen_US
dc.subject.meshGlycoproteins --metabolismen_US
dc.subject.meshHemagglutinins --metabolismen_US
dc.subject.meshMolecular Weighten_US
dc.titleMajor bovine brain stem glycoproteins sugar-specifically recognized by endogenous 14 kDa galactose-binding lectin.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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