Apoptosis gene reprograming of human peripheral blood mononuclear cells induced by radioiodine-131 (131I) irradiation

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dc.contributor.authorLi, JFen_US
dc.contributor.authorXie, LJen_US
dc.contributor.authorQin, LPen_US
dc.contributor.authorLiu, YFen_US
dc.contributor.authorZhang, TJen_US
dc.contributor.authorHuang, Yen_US
dc.contributor.authorCheng, MHen_US
dc.date.accessioned2020-04-10T01:42:39Z
dc.date.available2020-04-10T01:42:39Z
dc.date.issued2019-05
dc.description.abstractBackground & objectives: The nature of adaptable change of B-cell lymphoma-2 (BCL-2) and/or Bcl2-associated X protein (BAX) gene expression in the human peripheral blood mononuclear cells (PBMCs) irradiated by radioiodine in thyroid diseases therapy is not fully understood. In this study, the alternation of apoptotic gene expression was evaluated while the PBMCs collected from healthy volunteers were irradiated by the radioiodine-131 (131I). Methods: Fasting blood samples were obtained from healthy volunteers. PBMCs from group 0 to 6 were incubated and exposed to different doses of 131I in cell suspension for 6, 12, 24 and 48 h. The apoptosis rates and expression of BCL-2 and BAX genes of PBMCs were examined. Results: The apoptosis rate in the human PBMCs was gradually enhanced after six hour irradiation. The values of BCL-2 and BAX gene expression in groups 1-6 were higher than in group 0 within 6 h of irradiation, and then, these were decreased gradually from 6 to 12 h. BCL-2 gene expression increased in groups 1-3 after 12 h irradiation, but there was no difference in groups 4-6. The ratio of BCL-2/BAX gene expression among groups 4-6 gradually decreased during the period from 6 to 12 h, and it was significantly lower than in the group 0 at 12, 24 and 48 h. Interpretation & conclusions: The expression of BCL-2 and BAX genes was initially upregulated following irradiation. Later, the balance of BCL-2/BAX genes expression was adjusted, and then, PBMCs underwent apoptosis at higher doses of radiation.en_US
dc.identifier.affiliationsDepartment of Nuclear Medicine, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, PR Chinaen_US
dc.identifier.affiliationsDepartment of Breast & Thyroid Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, PR Chinaen_US
dc.identifier.citationLi JF, Xie LJ, Qin LP, Liu YF, Zhang TJ, Huang Y, Cheng MH. Apoptosis gene reprograming of human peripheral blood mononuclear cells induced by radioiodine-131 (131I) irradiation. Indian Journal of Medical Research. 2019 May; 149(5): 627-632en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/195896
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber5en_US
dc.relation.volume149en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_1455_17en_US
dc.subjectApoptosisen_US
dc.subjectgene expression -131Ien_US
dc.subjectmononuclear cellen_US
dc.subjectradiationen_US
dc.titleApoptosis gene reprograming of human peripheral blood mononuclear cells induced by radioiodine-131 (131I) irradiationen_US
dc.typeJournal Articleen_US
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