Association of DNA pattern of metastatic lymph node with disease free survival in patients with intraoral squamous cell carcinoma.

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dc.contributor.authorDas, Satya Nen_US
dc.contributor.authorKhare, Pratimaen_US
dc.contributor.authorPatil, Asawarien_US
dc.contributor.authorPandey, Ravindra Men_US
dc.contributor.authorSingh, Manoj Ken_US
dc.contributor.authorShukla, Nootan Ken_US
dc.date.accessioned2005-09-28en_US
dc.date.accessioned2009-05-27T07:01:36Z
dc.date.available2005-09-28en_US
dc.date.available2009-05-27T07:01:36Z
dc.date.issued2005-09-28en_US
dc.description.abstractBACKGROUND AND OBJECTIVE: Intraoral squamous cell carcinoma (OSCC) is one of the common tobacco related cancers affecting Indian population. These tumours are slow growing, endophytic and are mostly well differentiated. Cervical lymph node is the common site of metastasis of these tumours. In most of the patients cervical lymph node metastasis rather than the primary tumour, affects prognosis. However, no reports are available on the DNA pattern of the metastatic lymph nodes in patients with intraoral squamous cell carcinoma. Therefore, the present study was undertaken to observe DNA pattern of primary tumours and their corresponding metastatic lymph nodes and its association with the clinicopathological parameters and prognosis. METHODS: DNA flow cytometry was successfully carried out on 68 paraffin embedded specimens of the primary tumours and their 22 corresponding metastatic cervical lymph nodes. The findings were evaluated for their possible association with clinicopathological features of the tumour and disease free survival of patients with intraoral carcinoma. RESULTS: Analysis of nuclear DNA patterns revealed 32 (47.0%) diploidy and 36 (52.9%) aneuploidy in primary tumours whereas metastatic lymph nodes showed 7 (31.8%) diploidy and 15 (68.1%) aneuploidy. The aneuploidy group in metastatic lymph node had significantly higher S phase fraction (SPF) (P<0.01) and poor histological grade (P<0.002) as compared to their counterparts with diploidy. DNA pattern of metastatic lymph node further showed a significant association with disease free survival in the log rank test. Aneuploidy and high SPF in metastatic lymph node was found to be associated with early recurrence while DNA pattern of the primary tumour did not show significant association with the disease free survival. INTERPRETATION AND CONCLUSION: It may be concluded that aneuploidy and high SPF in metastatic lymph node might be considered as an important discriminatory risk factor in patients with similarly staged intraoral squamous cell carcinoma.en_US
dc.description.affiliationDepartment of Biotechnology, All India Institute of Medical Sciences, New Delhi, India. satya n_das@hotmail.comen_US
dc.identifier.citationDas SN, Khare P, Patil A, Pandey RM, Singh MK, Shukla NK. Association of DNA pattern of metastatic lymph node with disease free survival in patients with intraoral squamous cell carcinoma. Indian Journal of Medical Research. 2005 Sep; 122(3): 216-23en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/18979
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAdulten_US
dc.subject.meshCarcinoma, Squamous Cell --geneticsen_US
dc.subject.meshDNA --isolation & purificationen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphatic Metastasis --geneticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMouth Neoplasms --pathologyen_US
dc.subject.meshPloidiesen_US
dc.subject.meshSurvival Analysisen_US
dc.titleAssociation of DNA pattern of metastatic lymph node with disease free survival in patients with intraoral squamous cell carcinoma.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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