Trichinella spiralis-specific monoclonal antibodies and affinity-purified antigen-based diagnosis.

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dc.contributor.authorSrimanote, Pen_US
dc.contributor.authorIttiprasert, Wen_US
dc.contributor.authorSermsart, Ben_US
dc.contributor.authorChaisri, Uen_US
dc.contributor.authorMahannop, Pen_US
dc.contributor.authorSakolvaree, Yen_US
dc.contributor.authorTapchaisri, Pen_US
dc.contributor.authorMaleewong, Wen_US
dc.contributor.authorKurazono, Hen_US
dc.contributor.authorHayashi, Hen_US
dc.contributor.authorChaicumpa, Wen_US
dc.date.accessioned2009-05-27T17:08:24Z
dc.date.available2009-05-27T17:08:24Z
dc.date.issued2000-03-28en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractHybridomas secreting monoclonal antibodies (MAbs) to Trichinella spiralis were produced. Myeloma cells were fused with splenocytes of a mouse immunized with excretory-secretory (E-S) antigen of infective larvae. A large percentage of growing hybrids secreted antibodies cross-reactive to many of 23 heterologous parasites tested. Only 6 monoclones (designated 3F2, 5D1, 10F6, 11E4, 13D6 and 14D11) secreted MAbs specific to the E-S antigen and/or a crude extract (CE) of T. spiralis infective larvae. The 6 monoclones secreted IgM, IgG3, IgM, IgG3, IgG3 and IgG3, respectively. Clone 5D1 was selected to mass produce MAbs which were then coupled to CNBr-activated Sepharose CL-4B to prepare an affinity-purified antigen. Dot-blot ELISA with either purified antigen or CE was evaluated. There were 17 patients with acute trichinellosis and 76 individuals convalescing from T. spiralis infection (group 1). Controls were 170 patients with parasitic infections other than trichinellosis (group 2) and 35 healthy parasite-free controls (group 3). CE-ELISA was positive in all group 1 patients. However, sera from many group 2 patients also were reactive (opisthorchiasis-44.2%, schistosomiasis-44%, gnathostomiasis-30%, paragonimiasis-28.6%, taeniasis-27.3%, strongyloidiasis-23.1% and hookworm infections-20%). Affinity-purified antigen was 100% specific, all sera from group 2 and group 3 individuals tested negative. Although 74 of 76 patients (97.4%) with convalescing trichinellosis tested positive, sera from only 3 of 17 patients (17.6%) with acute T. spiralis were reactive. Thus, CE antigen is appropriate when sensitivity is needed, while purified antigen should be used when specificity is required. Dot-blot ELISA is easier to perform, more rapid and less expensive than indirect ELISA. Many samples can be assayed simultaneously, special equipment is not required, and results can be preserved for retrospective analysis. Dot-blot ELISA is therefore the method of choice for the rapid diagnosis of trichinellosis, particularly when more complex laboratory tests are unavailable.en_US
dc.description.affiliationDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400 Thailand.en_US
dc.identifier.citationSrimanote P, Ittiprasert W, Sermsart B, Chaisri U, Mahannop P, Sakolvaree Y, Tapchaisri P, Maleewong W, Kurazono H, Hayashi H, Chaicumpa W. Trichinella spiralis-specific monoclonal antibodies and affinity-purified antigen-based diagnosis. Asian Pacific Journal of Allergy and Immunology. 2000 Mar; 18(1): 37-45en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36537
dc.language.isoengen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Helminth --blooden_US
dc.subject.meshAntibodies, Monoclonalen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshAntigens, Helminth --isolation & purificationen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshChromatography, Affinityen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshHybridomas --immunologyen_US
dc.subject.meshImmunologic Testsen_US
dc.subject.meshMiceen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshTrichinella spiralis --immunologyen_US
dc.subject.meshTrichinosis --diagnosisen_US
dc.titleTrichinella spiralis-specific monoclonal antibodies and affinity-purified antigen-based diagnosis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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