Diagnosis of opisthorchiasis by enzyme-linked immunosorbent assay using partially purified antigens.

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dc.contributor.authorPoopyruchpong, Nen_US
dc.contributor.authorViyanant, Ven_US
dc.contributor.authorUpatham, E Sen_US
dc.contributor.authorSrivatanakul, Pen_US
dc.date.accessioned2009-05-27T17:08:21Z
dc.date.available2009-05-27T17:08:21Z
dc.date.issued1990-06-01en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractOpisthorchis viverrini antigens were partially purified from adult worms collected from liver and extrahepatic biliary system of infected hamsters. Tegument fraction was obtained by chemical extraction, whereas other fractions were purified by Sephadex G-200 gel filtration chromatography. Five fractions of O. viverrini antigens were obtained, namely tegument extract, somatic extract, fraction 1 (P1), fraction 2 (P2) and fraction 3 (P3), respectively. The enzyme-linked immunosorbent assay technique was used to compare the reactivity of the five partially purified antigens. The sensitivity and specificity of all five antigens were compared by testing against the sera of 78 O. viverrini-infected individuals from O. viverrini endemic areas and 70 individuals from non-endemic areas infected with hookworm, Trichuris and Ascaris including 49 individuals with negative stool examination. The assays performed with tegument extract, somatic extract and P1 fraction were found to have 100% sensitivity, whereas the sensitivities of those with P2 and P3 were 96.1% and 83.3%, respectively. The tegument extract had the highest specificity as demonstrated by the lowest cross-reactivity with other parasites. Our results indicated that surface tegument is the most suitable antigen for use in immunological diagnosis of opisthorchiasis.en_US
dc.description.affiliationDepartment of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationPoopyruchpong N, Viyanant V, Upatham ES, Srivatanakul P. Diagnosis of opisthorchiasis by enzyme-linked immunosorbent assay using partially purified antigens. Asian Pacific Journal of Allergy and Immunology. 1990 Jun; 8(1): 27-31en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36535
dc.language.isoengen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Helminth --analysisen_US
dc.subject.meshAntigens, Helminth --isolation & purificationen_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHumansen_US
dc.subject.meshOpisthorchiasis --diagnosisen_US
dc.subject.meshOpisthorchis --immunologyen_US
dc.subject.meshThailanden_US
dc.titleDiagnosis of opisthorchiasis by enzyme-linked immunosorbent assay using partially purified antigens.en_US
dc.typeJournal Articleen_US
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