The amino-terminal domain of human signal transducers and activators of transcription 1: overexpression, purification and characterization.

Simple item page
dc.contributor.authorPrabhu, Aratien_US
dc.contributor.authorCoutinho, Evansen_US
dc.contributor.authorSrivastava, Sudhaen_US
dc.date.accessioned2005-12-03en_US
dc.date.accessioned2009-06-01T14:46:17Z
dc.date.available2005-12-03en_US
dc.date.available2009-06-01T14:46:17Z
dc.date.issued2005-12-03en_US
dc.description.abstractThe dual functional signal transducers and activators of transcription (STAT) proteins are latent cytoplasmic transcription factors that play crucial roles in host defense. Animals that lack these proteins are highly susceptible to microbial and viral infections and chemically induced primary tumours. We have over expressed the amino-terminal domain of human STAT1 (hSTAT1) in Escherichia coli and purified it by affinity chromatography and gel filtration chromatography. The entire process has been monitored by gel electrophoresis. The pure protein has been characterized by mass spectrometry and 2-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy. Our results indicate that the N-terminus of hSTAT1 exists as a dimer in solution.en_US
dc.description.affiliationDepartment of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, Mumbai 400 098, India.en_US
dc.identifier.citationPrabhu A, Coutinho E, Srivastava S. The amino-terminal domain of human signal transducers and activators of transcription 1: overexpression, purification and characterization. Journal of Biosciences. 2005 Dec; 30(5): 611-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111065
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshChromatography, Affinity --methodsen_US
dc.subject.meshChromatography, Liquid --methodsen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gel --methodsen_US
dc.subject.meshEscherichia coli --geneticsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGlutathione Transferase --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNuclear Magnetic Resonance, Biomolecular --methodsen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshProtein Structure, Secondaryen_US
dc.subject.meshSTAT1 Transcription Factor --biosynthesisen_US
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization --methodsen_US
dc.titleThe amino-terminal domain of human signal transducers and activators of transcription 1: overexpression, purification and characterization.en_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Journal of Biosciences