GalNAc-T14 may be involved in regulating the apoptotic action of IGFBP-3.
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Date
2009-09
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Abstract
Insulin-like growth factor binding protein-3 (IGFBP-3) is known to induce apoptosis in an insulin-like growth factor
(IGF)-dependent and IGF-independent manner, but the mechanism underlying the IGF-independent effects remains
unclear. Polypeptide N-acetylgalactosaminyltransferase 14 (GalNAc-T14) is a novel IGFBP-3 binding partner. In this
paper, small interference RNA (siRNA) targeting GalNAc-T14 was used to examine whether GalNAc-T14 affects
the apoptotic action of IGFBP-3. Using semi-quantitative reverse-transcriptase polymerase chain reaction (RT-PCR)
and western blot analysis, we determined that GalNAc-T14 expression was downregulated by the siRNA directed
against GalNAc-T14. Apoptosis analysis of IGFBP-3-overexpressing cells treated with siRNA against GalNAc-
T14 was performed to determine if GalNAc-T14 was specifi cally involved in IGFBP-3 signalling. The results, as
determined by fl ow cytometric analysis and caspase-3 assay, showed that the extent of apoptosis induced by IGFBP-3
increased with RNA interference (RNAi) knockdown of GalNAc-T14. Our data suggest that GalNAc-T14 infl uences
the apoptotic action of IGFBP-3 and might mediate the signalling pathway of IGFBP-3. Experiments to determine the
role of GalNAc-T14 in the regulation of apoptosis induced by IGFBP-3 are under way.
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Wu Chen, Shan Yaojun, Liu Xinxia, Song Wenqian, Wang Jiali, Zou Minji, Wang Min, Xu Donggang. GalNAc-T14 may be involved in regulating the apoptotic action of IGFBP-3. Journal of Biosciences. 2009 Sep; 34(3): 389-395.