Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 analyzer – An Institutional Experience from North India.

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dc.contributor.authorSehgal, Sen_US
dc.contributor.authorKhan, Sen_US
dc.contributor.authorJetley, Sen_US
dc.contributor.authorAlvi, Y.en_US
dc.date.accessioned2023-07-14T06:58:51Z
dc.date.available2023-07-14T06:58:51Z
dc.date.issued2022-06
dc.description.abstractBackground: High performance liquid chromatography (HPLC) is the most commonly used method for detection and quantitative estimation of hemoglobin variants. Hemoglobinopathies are amongst the most common genetically inherited disorders, however, the exact magnitude of different hemoglobinopathies is obscure in India. This study was done with the aim of analyzing the different findings in HPLC using D-10 analyzer and evaluating the spectrum of different hemoglobin disorders in a hospital-based population of South Delhi. Such a prevalence study would be useful to review the various strategies that can be implemented for effective control and prevention of these disorders. Methods: A hospital based descriptive observational study was conducted in which all OPD and IPD patients who were advised HPLC during their clinical workup were included. Analysis of EDTA blood samples was done by Bio Rad D10 Dual program HPLC instrument. The exact percentage of HbA, HbA2, HbF and any other variant hemoglobin was estimated. Presumptive identification of hemoglobin variants was made primarily by their percentage, retention time (RT) and peak characteristics. HPLC findings were correlated with the clinical history, family history and the CBC and peripheral smear findings in all cases. Results: On HPLC analysis, 79% of the patients had no abnormality detected and the report was within normal limits. The commonest hemoglobinopathy was Beta Thalassemia Trait followed by HbE trait. The other hemoglobinopathies detected were HbD Punjab Heterozygous (3 cases, 0.5%), Beta thalassemia homozygous (3 cases, 0.5%), Sickle cell Heterozygous (2 cases, 0.3%), HbJ Meerut Heterozygous (2 cases, 0.3%). One case each of Sickle cell Homozygous (0.15%), Compound Heterozygous HbS/beta thalassemia trait (0.15%), HbE Homozygous (0.15%), Compound Heterozygous HbE/beta thalassemia trait (0.15%), and Homozygous delta beta thalassemia (0.15%) were also diagnosed. Conclusion: This study gives an important insight to the present day scenario of hemoglobinopathies in patients in South Delhi in relation to the hematological profile. It highlights the chromatogram findings of different hemoglobinopathies on the D10 analyzer. The comprehensive data obtained by such series can help in the formulation and development of infrastructure and policies for hemoglobinopathy prevention, diagnosis and management.en_US
dc.identifier.affiliationsDepartment of Pathologyen_US
dc.identifier.affiliationsDepartment of Pathologyen_US
dc.identifier.affiliationsDepartment of Pathologyen_US
dc.identifier.affiliationsDepartment of Community Medicine, Hamdard Institute of Medical Sciences and Research, New Delhien_US
dc.identifier.citationSehgal S, Khan S, Jetley S, Alvi Y.. Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 analyzer – An Institutional Experience from North India.. Light House Polyclinic. 2022 Jun; 21(2): 1-5en_US
dc.identifier.issn0972-5997
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/218304
dc.languageenen_US
dc.publisherLight House Polyclinicen_US
dc.relation.issuenumber2en_US
dc.relation.volume21en_US
dc.source.urihttps://www.ojhas.org/issue82/2022-2-10.pdfen_US
dc.subjectHemoglobin varianten_US
dc.subjectHemoglobinopathyen_US
dc.subjectD-10 analyzeren_US
dc.subjectNorth Indiaen_US
dc.titleEvaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 analyzer – An Institutional Experience from North India.en_US
dc.typeJournal Articleen_US
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