Isolation and characterization of diethyl phthalate degrading bacteria from crude oil contaminated soil

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dc.contributor.authorÇevik, P.K.en_US
dc.contributor.authorEro?lu, A.B.en_US
dc.contributor.authorYildizli, G.en_US
dc.contributor.authorCo?an, D.en_US
dc.contributor.authorKantar, Ç.en_US
dc.contributor.authorCoral, G.en_US
dc.date.accessioned2020-11-18T10:16:13Z
dc.date.available2020-11-18T10:16:13Z
dc.date.issued2019-05
dc.description.abstractAim: The aim of this study was to isolate, identify and characterize diethyl phthalate degrading bacterial strains isolated from a crude oil contaminated soil from a landfill dump site of a petroleum refinery in Mersin, Turkey. Methodology: The bacteria was isolated from a crude oil contaminated soil and characterized by 16S rRNA analysis. Bacterial genomic DNA was identified by 16S rRNA analyses. Biodegredation experiments were conducted for 5 days and plasmid curing experiment was performed. Catechol test was carried out to determine phthalate degradation pathways. Results: The isolated bacteria from soil were identified as Pseudomonas putida based on 16S rRNA sequences. The size of the plasmid was estimated to be about 15.9 kb. Results of biodegradation experiments indicated that the diethyl phthalate concentrations were reduced by 85.5% after 5 days of incubation at pH 7.0 and 30°C. The ability of P. putida degrading diethyl phthalate was found to be plasmid-mediated through curing experiments. Interpretation: The study suggested that plasmid of Pseudomonas putida PAG5 could be involved in effective degradation of diethyl phthalateen_US
dc.identifier.affiliationsDepartment of Biology, Faculty of Science and Letters, Mersin University, Mersin, 33343, Turkeyen_US
dc.identifier.affiliationsDepartment of Biotechnology, Faculty of Science and Letters, Mersin University, Mersin, 33343, Turkeyen_US
dc.identifier.affiliationsDepartment of Biotechnology, Faculty of Science and Letters, Mersin University, Mersin, 33343, Turkeyen_US
dc.identifier.affiliationsDepartment of Biology, Faculty of Science and Letters, Mersin University, Mersin, 33343, Turkeyen_US
dc.identifier.affiliationsDepartment of Environmental Engineering, Faculty of Engineering, Çanakkale Onsekiz Mart University, Çanakkale, 17100, Turkeyen_US
dc.identifier.affiliationsDepartment of Biotechnology, Faculty of Science and Letters, Mersin University, Mersin, 33343, Turkeyen_US
dc.identifier.citationÇevik P.K., Ero?lu A.B., Yildizli G., Co?an D., Kantar Ç., Coral G.. Isolation and characterization of diethyl phthalate degrading bacteria from crude oil contaminated soil. Journal of Environmental Biology . 2019 May; 40(3): 275-282en_US
dc.identifier.issn0254-8704
dc.identifier.issn2394-0379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214550
dc.languageenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.issuenumber3en_US
dc.relation.volume40en_US
dc.source.urihttps://www.jeb.co.in/journal_issues/201905_may19/paper_02.pdfen_US
dc.subjectBiodegradationen_US
dc.subjectDiethyl phthalateen_US
dc.subjectPhthalate estersen_US
dc.subjectPlasmiden_US
dc.subjectPseudomonas putidaen_US
dc.titleIsolation and characterization of diethyl phthalate degrading bacteria from crude oil contaminated soilen_US
dc.typeJournal Articleen_US
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