Prenatal diagnosis of alpha-thalassemia-1 (SEA type) by chorionic villus sampling.
dc.contributor.author | Chanprapaph, Pharuhas | en_US |
dc.contributor.author | Tongsong, Theera | en_US |
dc.contributor.author | Wanapirak, Chanane | en_US |
dc.contributor.author | Sirichotiyakul, Supattra | en_US |
dc.contributor.author | Sanguansermsri, Torpong | en_US |
dc.date.accessioned | 2009-05-27T18:33:57Z | |
dc.date.available | 2009-05-27T18:33:57Z | |
dc.date.issued | 2002-10-28 | en_US |
dc.description | Chotmaihet Thangphaet. | en_US |
dc.description.abstract | OBJECTIVE: To describe the experience of prenatal diagnosis for Hb Bart's disease, by chorionic villus sampling (CVS) with DNA analysis. DESIGN: Descriptive study SETTINGS: Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University. SUBJECTS: Sixteen high risk pregnancies at risk of Hb Bart's disease who were eligible for CVS criteria between 1 January, 1999 and May 31, 2000. MATERIAL AND METHOD: Fetal villi were obtained by either transcervical (TC) or transabdominal (TA) CVS route to extract DNA and detect for alpha-thal-1 gene deletion (SEA type) with modified Chang's method. The CVS results were confirmed by either serial ultrasound or cordocentesis or diagnosis after pregnancy termination. MAIN OUTCOME MEASURES: The efficacy, safety and pregnancy outcomes. RESULTS: CVS was successfully done in all of 16 cases (5 with TC and 11 with TA), The mean gestational age was 13.25 +/- 2.9 weeks. The procedure time for TA was shorter than that of TC (4.64 +/- 5.4 vs 10.4 +/- 11.3 min). The CVS result showed as follows: 3 normal fetuses, 7 alpha-thal-1 carriers, 4 fetal Hb Bart's, 1 misdiagnosis and 1 failure to diagnosis due to technical error. The sensitivity and specificity were 100 per cent (4/4) and 90.91 per cent (10/11), respectively. One case of Hb Bart's misdiagnosis and one failure case were later confirmed for alpha-thal-1 trait and alpha-thal-1/ Hb E trait by cordocentesis, respectively. The pregnancy outcomes included 11 livebirths, 4 terminated cases and 1 fetal loss of continuing pregnancies. No serious complications occurred. CONCLUSION: This preliminary experience suggests that CVS is an effective method for early prenatal diagnosis of fetal Hb Bart's. | en_US |
dc.description.affiliation | Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. | en_US |
dc.identifier.citation | Chanprapaph P, Tongsong T, Wanapirak C, Sirichotiyakul S, Sanguansermsri T. Prenatal diagnosis of alpha-thalassemia-1 (SEA type) by chorionic villus sampling. Journal of the Medical Association of Thailand. 2002 Oct; 85(10): 1049-53 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/39403 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://www.mat.or.th/journal/all.php | en_US |
dc.subject.mesh | Chorionic Villi Sampling | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Fetal Diseases --diagnosis | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Polymerase Chain Reaction | en_US |
dc.subject.mesh | Pregnancy | en_US |
dc.subject.mesh | Pregnancy Outcome | en_US |
dc.subject.mesh | Pregnancy, High-Risk | en_US |
dc.subject.mesh | alpha-Thalassemia --diagnosis | en_US |
dc.title | Prenatal diagnosis of alpha-thalassemia-1 (SEA type) by chorionic villus sampling. | en_US |
dc.type | Journal Article | en_US |
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