Prenatal diagnosis of alpha-thalassemia-1 (SEA type) by chorionic villus sampling.

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dc.contributor.authorChanprapaph, Pharuhasen_US
dc.contributor.authorTongsong, Theeraen_US
dc.contributor.authorWanapirak, Chananeen_US
dc.contributor.authorSirichotiyakul, Supattraen_US
dc.contributor.authorSanguansermsri, Torpongen_US
dc.date.accessioned2009-05-27T18:33:57Z
dc.date.available2009-05-27T18:33:57Z
dc.date.issued2002-10-28en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractOBJECTIVE: To describe the experience of prenatal diagnosis for Hb Bart's disease, by chorionic villus sampling (CVS) with DNA analysis. DESIGN: Descriptive study SETTINGS: Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University. SUBJECTS: Sixteen high risk pregnancies at risk of Hb Bart's disease who were eligible for CVS criteria between 1 January, 1999 and May 31, 2000. MATERIAL AND METHOD: Fetal villi were obtained by either transcervical (TC) or transabdominal (TA) CVS route to extract DNA and detect for alpha-thal-1 gene deletion (SEA type) with modified Chang's method. The CVS results were confirmed by either serial ultrasound or cordocentesis or diagnosis after pregnancy termination. MAIN OUTCOME MEASURES: The efficacy, safety and pregnancy outcomes. RESULTS: CVS was successfully done in all of 16 cases (5 with TC and 11 with TA), The mean gestational age was 13.25 +/- 2.9 weeks. The procedure time for TA was shorter than that of TC (4.64 +/- 5.4 vs 10.4 +/- 11.3 min). The CVS result showed as follows: 3 normal fetuses, 7 alpha-thal-1 carriers, 4 fetal Hb Bart's, 1 misdiagnosis and 1 failure to diagnosis due to technical error. The sensitivity and specificity were 100 per cent (4/4) and 90.91 per cent (10/11), respectively. One case of Hb Bart's misdiagnosis and one failure case were later confirmed for alpha-thal-1 trait and alpha-thal-1/ Hb E trait by cordocentesis, respectively. The pregnancy outcomes included 11 livebirths, 4 terminated cases and 1 fetal loss of continuing pregnancies. No serious complications occurred. CONCLUSION: This preliminary experience suggests that CVS is an effective method for early prenatal diagnosis of fetal Hb Bart's.en_US
dc.description.affiliationDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.en_US
dc.identifier.citationChanprapaph P, Tongsong T, Wanapirak C, Sirichotiyakul S, Sanguansermsri T. Prenatal diagnosis of alpha-thalassemia-1 (SEA type) by chorionic villus sampling. Journal of the Medical Association of Thailand. 2002 Oct; 85(10): 1049-53en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/39403
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshChorionic Villi Samplingen_US
dc.subject.meshFemaleen_US
dc.subject.meshFetal Diseases --diagnosisen_US
dc.subject.meshHumansen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshPregnancyen_US
dc.subject.meshPregnancy Outcomeen_US
dc.subject.meshPregnancy, High-Risken_US
dc.subject.meshalpha-Thalassemia --diagnosisen_US
dc.titlePrenatal diagnosis of alpha-thalassemia-1 (SEA type) by chorionic villus sampling.en_US
dc.typeJournal Articleen_US
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