Replication, maturation and physical mapping of bacteriophage MB78 genome.
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Date
1991-09
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Abstract
Bacteriophage MB78 is a virulent phage of Salmonella typhimurium. The viral
DNA is 42 kb in size and seems to be circularly permuted. We show that viral DNA
replication is through concatemeric DNA formation which is subsequently converted into
full length DNA through headful packaging. A restriction map of MB78 DNA for six
restriction endonucleases e.g. BgIII, PvuII, ECORI, ClaI, SalI and SmaI has been
constructed. The yield of certain fragments in less than molar amount is explained in terms
of permutation and the headful mechanism of packaging. The packaging site (pac site) has
been suggested.
Description
Keywords
Bacteriophage M B78, Salmonella phage, restriction map, DNA packaging, 'pac' site
Citation
Khan Saeed A, Murty S S, Zargar Manzoor A, Chakravorty M. Replication, maturation and physical mapping of bacteriophage MB78 genome. Journal of Biosciences. 1991 Sept; 16(3): 161-174.