Qualitative analyses of lignocellulolytic enzymes produced by Emericella sp strain HST9 under diverse culture conditions

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dc.contributor.authorTanvien_US
dc.contributor.authorGoyal, S.en_US
dc.contributor.authorDhankar, R.en_US
dc.contributor.authorChaudhary, S.en_US
dc.contributor.authorNandnien_US
dc.contributor.authorDevi, Sen_US
dc.date.accessioned2020-11-18T10:16:06Z
dc.date.available2020-11-18T10:16:06Z
dc.date.issued2019-11
dc.description.abstractAim: Optimization of cultural conditions for improved lignocellulolytic enzyme production by an ascomycete HST9 isolated from leaf and litter waste. Methodology: The fungus HST9 was isolated from leaf and litter waste collected from Chaudhary Charan Singh Haryana Agricultural University, Hisar, India. Culture experiments were conducted at different temperature, pH, incubation periods and aeration conditions. Effects of addition of different concentrations of various metal ions, different carbon complexes and nitrogen salts on enzyme production were also studied under submerged culture condition. Enzyme activities were measured by standard protocols using spectrophotometer. Results: HST9 grew well and produced optimum enzymes at 30oC on 7th day of incubation at stationary conditions and pH- 6. Overall enzyme activities decreased after addition of metal salts. Carboxymethyl cellulose (300 mg l-1) and alkali lignin (200 mg l-1) were observed to be the best carbon complexes for cellulolytic and ligninolytic activities. Ammonium sulfate was found to be a better nitrogen source compared to others. Under optimum conditions, different enzyme activities observed were 0.011 IU m l-1 FPase, 0.015 IU ml-1 CMCase, 6.5 IU m l-1 Lac, 57.5 IU m l-1 LiP and 4 IU ml-1 MnP. Molecular phylogenetic analysis of the strain confirmed that strain HST9 showed closeness with genus Emericella. Interpretation: Lignocellulolytic enzyme activity of Emericella isolate HST9 enhanced at optimum culture conditions, signifying that it can be used as a biological agent to degrade lignocellulosic waste.en_US
dc.identifier.affiliationsDepartment of Microbiology,CCS Haryana Agricultural University,Hisar-125 004, Indiaen_US
dc.identifier.citationTanvi, Goyal S., Dhankar R., Chaudhary S., Nandni, Devi S. Qualitative analyses of lignocellulolytic enzymes produced by Emericella sp strain HST9 under diverse culture conditions. Journal of Environmental Biology . 2019 Nov; 40(6): 1211-1218en_US
dc.identifier.issn0254-8704
dc.identifier.issn2394-0379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214460
dc.languageenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.issuenumber6en_US
dc.relation.volume40en_US
dc.source.urihttps://www.jeb.co.in/journal_issues/201911_nov19/paper_12.pdfen_US
dc.subjectAscomycetesen_US
dc.subjectComposten_US
dc.subjectEmericella spen_US
dc.subjectEndoglucanasesen_US
dc.subjectLignocelluloseen_US
dc.titleQualitative analyses of lignocellulolytic enzymes produced by Emericella sp strain HST9 under diverse culture conditionsen_US
dc.typeJournal Articleen_US
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