Update Ayurveda 2014 Abstracts Dr. Sharadini Dahanukar Prize Session.
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2015-05
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Abstract
A comparative study of Curcuma longa and its fortified
varieties on glucose uptake in normal and insulin
resistant cells
Vaishali Dongare, Renuka Munshi, Samidha Joshi,
Supriya Bhalerao
Department of Clinical Pharmacology, TN Medical College and BYL
Nair Hospital, Mumbai, Maharashtra, India
E-mail: vaishali.dongare@ymail.com
Background: Introduction of metal wire into the tuber of
Curcuma longa plant can prove a novel way to use metals
to get their therapeutic benefits in Ayurveda. Objective: The
aim was to evaluate the effect of C. longa and its fortified
varieties on glucose uptake in naïve and insulin resistance
3T3L1 cells. Materials and Methods: Zinc (Zn) and Tin (Sn)
metal wire was inserted into the tuber of growing C. longa
plant, which was further used as a therapeutic agent and
glucose uptake activity was analyzed by measuring uptake
of radio‑labeled glucose in 3T3‑L1 adipocytes, in naïve
and insulin resistant state. Results: At the end of 20 min
fortified variety of Sn showed dose dependent increase
with maximum effect at 50 μg/ml as compared to fortified
variety of Zn and control variety that is, C. longa alone. At
the end of 24 h both the fortified varieties exhibited lesser
glucose uptake when compared to the control variety. In
resistant state, glucose uptake was higher in all the groups
when compared with dexamethasone treated adipocytes.
However, the effect with a fortified variety was lesser than
control variety. Conclusion: Insertion of metals (Zn and Sn)
into the tubers did not enhance the glucose uptake activity
known to be exhibited by C. longa. The present study
needs to be explored further in terms of insertion technique,
concentration of metals and climatic conditions.
UA/PZ/02
An experimental study to evaluate the anti‑osteoporotic
effect of Panchtikta ghrita on glucocorticoid‑induced
osteoporosis rat model
Bhagyeshri Rane, Tanvi Patil, Renuka Munshi,
Supriya Bhalerao, Chetan Gurada
Department of Clinical Pharmacology, TN Medical College and BYL
Nair Hospital, Mumbai, Maharashtra, India
E‑mail: bnrane87@gmail.com
Background: Although the exact prevalence of steroid‑induced
osteoporosis remains unclear, it is estimated, that significant
bone loss occurs in 30–50% of patients receiving
chronic, long‑term steroid therapy. Objective: The study
was conducted to develop the glucocorticoid‑induced
osteoporosis (GC) model in Sprague Dawley weanling
rats using different doses of methylprednisolone (MP)
and evaluate the anti‑osteoporotic effect of an Ayurvedic
formulation, Panchatikta ghrita [PG], in this model.
Materials and Methods: Institutional Animal Ethics
Committee approval was obtained. Development of the
model was done by subcutaneous injection of two doses
of MP (14 mg/kg/week and 28 mg/kg/week) for 4 weeks
in 21 day old weanlings. The standard drug (alendronate:
2.9 mg/kg/day) and test drug (PG 1.35 g/kg/day) were
administered orally 2 weeks after MP (14 mg/kg/week)
injection and continued for 4 weeks. Serum and urine
calcium and inorganic phosphate were analyzed at weekly
intervals. Animals were sacrificed after 6 weeks, and
femur bones were processed to measure bone hardness
and elasticity and for histological studies. Results: Phase
I: As rats treated with MP 14 mg/kg/week showed
optimum osteoporotic effect with no mortality when
compared to MP 28 mg/kg/week, this dose was used
in the second phase. Osteoporotic rats treated with PG
1.35 g/kg showed increase in serum calcium and phosphate
levels (10.0 ± 0.59, 8.36 ± 0.26) while urine calcium
and phosphate levels (2.78 ± 0.74, 278.6 ± 45.5) were
significantly reduced. A significant decrease in number of
osteoclasts (1.0 ± 0.63, 0.83 ± 0.40, 0.83 ± 0.40) while an
increase in bone hardness and elasticity (203.03 ± 54.15,
5.51 ± 2.72) were observed as compared to diseased
group (184.25 ± 100.21), demonstrating anti‑osteoporotic
effect of PG. Conclusion: Based on its effect on restoration
of mineral content, reduction in osteoclasts and increase
in bone hardness, it can be concluded that PG has an
anti‑osteoporotic effect in GC‑induced osteoporosis model.
UA/PZ/03
Androgenic activity of Suvarna bhasma evident as
changes in the cytoarchitecture of the testes, seminal
vesicles, and ventral prostate
Gaurav Vaish, Pawankumar Godatwar
Department of Roga and Vikriti Vijnana, National Institute of Ayurveda,
Jaipur, Rajasthan, India
E‑mail: gauravayurveda@rediffmail.com
Background: The presence of gold in normal human seminal
plasma has been reported. In some cases of pathological
semen samples, the value of gold was less than the normal,
possibly demonstrating the need of gold for the fertility
potential. Objective: To assess the role of gold for possible
androgenic activity which would be evident as changes in the
cytoarchitecture of the testes, seminal vesicles and ventral
prostate and to assess the effect of gold on sperm count and
motility. Materials and Methods: The study was carried out
in two groups of albino rats. After recording the initial data,
five rats in both the groups A and B were started with lactose
solution and Suvarna bhasma suspension therapy for 15 days.
Cauda epididymis suspension was examined for sperm count,
motility and morphology. Dissected testes, seminal vesicles,
and prostate were subjected to microscopic examination for
cytoarchitecture. Results: Following treatment by S. bhasma, concentration of spermatozoa in cauda epididymis of the rat
increased significantly, the percentage of the rapid linear
progressive motility of spermatozoa in rats also increased. The
area of interstitial tissue of the testes increased significantly,
and the diameter of seminiferous tubules in rat testis was
reduced while the number of seminiferous tubules per field in
rat testis was increased. Conclusion: S. Bhasma is effective
on sperm count and motility and showed androgenic activity,
which was evident as changes in the cytoarchitecture of the
testes, seminal vesicles, and ventral prostate.
UA/PZ/04
Unmadgajakesari: A novel antipsychotic drug
Rajeeta Joseph, Vijaya Pandit1, Asmita Wele2
Department of Pharmacology, B.V.D.U. Dental College and
Hospital, 1Department of Pharmacology, B.V.D.U Medical College,
2Department of Rasashastra, B.V.D.U. College of Ayurveda, Pune,
Maharashtra, India
E‑mail: rajeetajoseph@gmail.com
Background: Schizophrenia is a complex neuropsychiatric
disorder with abnormalities involving multiple neurotransmitters
like dopamine, serotonin, glutamate and gamma‑aminobutyric
acid (GABA). Unmadgajakesari (UGK) is a herbomineral
formulation claimed to possess antipsychotic activity. Hence, the
present study was carried out to assess the antipsychotic profile
of UGK. Objective: To evaluate the antipsychotic activity of UGK
in animal models. Materials and Methods: After performing acute
toxicity studies of UGK, it was evaluated for its dopaminergic,
serotonergic, N‑methyl‑D‑aspartate (NMDA) and GABA activity
in animal models viz., inhibition of apomorphine‑induced
climbing (dopamine), Inhibition of 5‑HTP‑induced head
twitches (serotonin), antagonism of MK‑801‑induced
hyperlocomotion (NMDA) in mice and antagonism of PTZ‑induced
convulsion (GABA) in rats. Animals were divided into 6 groups
for studying each neurotransmitter. Each group comprised of
six animals wherein Group I ‑ Normal control, Group II ‑ Vehicle
control (ghrita), Group III ‑ Drug control (positive control). In test
groups (IV‑VI) UGK were administered in doses of 100, 200 and
400 mg/kg in mice and 70, 140 and 280 mg/kg in rats. All the
drugs were given orally for 8 days, and the study parameter
was evaluated on day 1 and 8. Results: UGK was found to be
nontoxic upto dose 2000 mg/kg. Significant antidopaminergic,
antiserotonergic, anti NMDA activity was obtained on day 1
with no significant increase till day 8. Efficacy of UGK was not
increased with dose increment. Significant gabergic activity was
seen on day 8 only in low dose. Conclusion: Lack of adverse
effects and multitude of actions exhibited by UGK may prove it
to be one of the novel drugs in the treatment of psychosis, not
responding to modern medicine.
UA/PZ/05
Study of a holistic approach of antenatal care on
pregnancy outcome
Supriya Gugale
Tanushree Garbhsanskar Center, Pune and Neel Hospital, Panvel,
Navi Mumbai, Maharashtra, India
E‑mail: doc_supriya13@yahoo.com
Background: Pregnancy is a unique state of physiological
stress. Studies show that very high level of stress may
contribute to an increased risk of premature delivery
or low birth weight babies, increase heart rate, blood
pressure. Objective: To study the holistic approach of
antenatal care to improve the gestational age at birth.
Materials and Methods: A total of 532 women attending the
antenatal clinic both at Tanushree Garbhsanskar Kendra Pune
and Neel Clinic, Panvel, Navi Mumbai, were enrolled between
18 and 28 week of pregnancy in prospective nonrandomized,
matched, interventional study. Totally 254 women were
enrolled in study group and 278 women in the control group.
Holistic antenatal care included physical, mental and spiritual
care. Stress management through Rajayoga Meditation (as
taught by Brahma Kumaris), Nutritious and Satwik food,
antenatal physical and breathing exercises were advised
for the study group. Exercises, walking and meditation
were practiced by the study group from date of entry until
delivery. The control group walked half an hour twice a day
(standard obstetric advice) during study period. Compliance
in both groups was ensured by frequent telephone calls
and maintenance of daily activity charts. Results: Preterm
labor was significantly lower in study group. The number
of babies with birth weight ≥2500 g were significantly
higher in study group. Vaginal delivery was significantly
higher in study group. Intrauterine growth restriction (IUGR)
and pregnancy‑induced hypertension with associated IUGR
were significantly lower in study group. Conclusion: Holistic
Antenatal care is safe, relatively cheap to implement and
would reduce the costs of long‑term health care.
UA/PZ/06
An Ayurvedic approach in the management of
Guillain–Barre syndrome: A case study
Amit Nakanekar, Sunanda Bhopale, Suraj Rathod,
Harshad Gulhane, J. D. Gulhane
Government Ayurved College, Nagpur, Maharashtra, India
E‑mail: amitnakanekar@gmail.com
Background: Guillain–Barre syndrome (GBS) is an acute,
frequently severe and fulminant polyradiculopathy that is
autoimmune in nature. GBS is a rare disorder that causes
immune systems to attack peripheral nervous system.
Objective: To observe the effect of Ayurvedic treatment in the
management of GBS. Materials and Methods: A 46‑year‑old
male patient, presenting with sudden onset, complete
paralysis of all four limbs (quadriplegia), unable to walk,
stand, sit, difficulty in deglutition (dysphagia) and dysarthria,
having Foley’s catheter and Ryle’s Tube was brought
by relative to OPD of our Institute. He was provisionally
diagnosed as subacute sensory motor paraplegia. Previously,
the patient admitted and treated in our institute but did
not show any sign of improvement and hence patient was
admitted and treated with Ayurvedic treatment. As per
Ayurvedic classics, this condition can be correlated with
Sarvangagatavatavyadhi (~Vata disorder affecting all parts
of the body), which is Apatarpana in nature (~diseases
with deprived nourishment of body tissue) preceded by
Jvara (~h/o fever before onset of GBS). Hence, the principle
of treatment is Santarpana Chikitsa (~nourishing treatment)
and jwaraghna chikitsa (~treatment of fever) using various
Ayurvedic herbomineral compounds. Results: Remarkable
results were observed in the form of improvement in the muscle power from 0 to 5 of all four limbs, improvement in
speech. Post treatment there was no difficulty in deglutition,
in sitting, standing and walking, and the patient’s condition
became near to normal movements. Conclusion: Ayurvedic
treatment has a beneficial effect in the management of GBS.
UA/PZ/07
Exploring multiscale entropy measures for the radial
pulse analysis with Traditional Indian Medicine
perspective for dominant humor identification
Prasad Joshi, Rohin Daruwala
Department of Electrical Engineering, Veermata
Jijabai Technological Institute, Matunga (East), Mumbai,
Maharashtra, India
E‑mail: prasad.joshi@djsce.ac.in
Background: Radial pulse analysis is an important tool
for patient diagnosis practiced in Traditional Indian
Medicinal (TIM) Science – Ayurveda. Objective: Objective
analysis and interpretation of radial pulse signal from signal
processing perspective to obtain measures for dominant
humor identification in healthy states has been attempted.
Materials and Methods: Electret‑based capacitance sensing
mechanism and instrumentation has been build and
implemented for sensing radial pulse perturbations. Sensed
signal is sampled at 500 Hz and digitized using 14‑bit data
acquisitions unit. Pulse signal from point position proximal
to the thumb, and along the radial artery, of the right/left
hand is identified for single‑channel pulse logging. Signals
for healthy individual have been logged from the indicated
position, in the three humor interval periods – 7 am,
1 pm and 5 pm corresponding to dominant humor Kapha,
Pitta and Vata for at‑least 5‑min over a period of 8 days.
Results: Radial pulse analysis, using multiscale permutation
entropy (MSPE), for dominant humor identification has been
implemented. Identifiably distinct trajectory variations, of
MSPE values for pulse signal in dominant humor interval
have been obtained. MSPE variations of Pitta (Pt) humor
displayed by control subject correlate with a factor of
0.72 and P value of 0.014 with those of similar age group
individuals. Conclusions: Objective evaluation of the radial
pulse signal for obtaining results similar to those arrived
by a trained TIM physician, using sense perception, can
contribute in the development of value added diagnostic tool.
UA/PZ/08
Effect of Withania somnifera on physical and
cardiovascular performance‑induced by physical stress
in healthy human volunteers: A pilot study
A. P. Petare, B. A. Salve, R. K. Tripathi, S. V. Kapse, A. Raut,
N. N. Rege
Department of Pharmacology and Therapeutics, Seth G.S. Medical
College and K.E.M. Hospital, Mumbai, Maharashtra, India
E‑mail: anuppetare@gmail.com
Background: Withania somnifera (WS) known as rejuvenator,
increases vigor, vitality, immunity was evaluated for
anti‑stress/adaptogenic activity in healthy volunteers
during exercise, versus placebo. Objectives: The primary
objective of this study was to evaluate effect of WS
on physical performance and secondary objectives
were to evaluate muscle power, maximal oxygen
consumption (VO2max) and sympathetic activity in
comparison with placebo when subjected to physical
stress. Materials and Methods: After Ethics committee
approval and consenting, 30 participants were randomly
assigned into three groups of 10 each namely placebo, WS
330 mg and WS 500 mg after screening visit for physical
fitness. They were assessed on baseline visit, day 14 and
day 28. Physical stressors used were three cycle ergometer
exercises, Jammers’ hand held dynamometer test and
cold pressor test in each visit. Physical performance was
evaluated as the maximum distance and speed, VO2max, hand
grip strength. Cardiovascular response was assessed by
multiple heart rate and blood pressure (BP) measurements
during each test. Repeated measure of ANOVA used for the
within group analysis, post‑hoc Tukey’s test and unpaired
t‑test used. Results: On day 28, WS 330 and WS 500 groups
showed a significant increase mean distance travelled and
mean average speed compared to placebo (P < 0.005).
WS 500 group showed significant decreased mean
systolic BP on fixed workload exercise, compared to
placebo (P < 0.005). Compared to baseline WS 330 and WS
500 groups showed significant increased VO2max and hand
grip strength (P < 0.005). Conclusion: WS demonstrated
increasing trend in tolerance to exercise from baseline
showing adaptogenic activity.
UA/PZ/09
Evaluation of Withania somnifera on ethanol
consumption and decision making in model of ethanol
dependence in mice: An experimental study
Kshitij S. Jadhav, Padmaja A. Marathe
Department of Pharmacology and Therapeutics, Seth GS medical
college, KEM hospital, Parel, Mumbai, Maharashtra, India
E‑mail: dr.kshitij@yahoo.com
Background: Therapy for alcohol addiction described in
modern medicine is modestly beneficial and having high
relapse rate. There is a need for more efficacious drugs.
Withania somnifera (WS) has been shown to inhibit
morphine tolerance and dependence in mice possibly due
to its gamma‑aminobutyric acid (GABA)‑mimetic action.
Earlier work in our laboratory showed that WS protected
against alcohol relapse and withdrawal. Depressed GABA
and serotonin neurotransmission increase ethanol intake and
impair decision making in addiction. WS has been shown
to enhance GABAergic and serotonergic transmission in
the CNS. Objectives: To evaluate effects of WS on ethanol
consumption and decision making in the experimental model
of ethanol dependence. Materials and Methods: Following
Animal Ethics Committee’s permission, 24 male
C57BL/6 mice were divided into four groups as follows:
Group 1: Vehicle, Group 2: 1 mg/kg/day naltrexone, Group 3:
WS‑390 mg/kg/day and Group 4: WS‑650 mg/kg/day.
Models of ethanol dependence (intermittent access) and
Iowa gambling task (IGT) were used. Variables measured
were ethanol and water intake/kg/day and percentage
ethanol preference for 15 days after induction of ethanol
dependence. IGT was performed for 9 days on the same
mice after first experiment and number of choices for
disadvantageous arm was noted. Data were analyzed set at P < 0.05. Results: WS (390 mg/kg) group showed
decreased ethanol intake whereas WS (650 mg/kg)
showed decreased ethanol intake, ethanol preference as
well as improved IGT performance (P < 0.05 vs. control);
comparable to naltrexone. Conclusions: WS effectively
decreased ethanol consumption and improved decision
making in ethanol dependent mice.
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Update Ayurveda 2014 Abstracts Dr. Sharadini Dahanukar Prize Session. Journal of Ayurveda and Integrative Medicine. 2015 May; 6(suppl_5): 1-4.