Residual ordered structure in denatured proteins and the problem of protein folding.
| dc.contributor.author | Basharov, Mahmud A | |
| dc.date.accessioned | 2012-08-09T10:41:45Z | |
| dc.date.available | 2012-08-09T10:41:45Z | |
| dc.date.issued | 2012-02 | |
| dc.description.abstract | Structural characteristics of numerous globular proteins in the denatured state have been reviewed using literature data. Recent more precise experiments show that in contrast to the conventional standpoint, proteins under strongly denaturing conditions do not unfold completely and adopt a random coil state, but contain significant residual ordered structure. These results cast doubt on the basis of the conventional approach representing the process of protein folding as a spontaneous transition of a polypeptide chain from the random coil state to the unique globular structure. The denaturation of proteins is explained in terms of the physical properties of proteins such as stability, conformational change, elasticity, irreversible denaturation, etc. The spontaneous renaturation of some denatured proteins most probably is merely the manifestation of the physical properties (e.g., the elasticity) of the proteins per se, caused by the residual structure present in the denatured state. The pieces of the ordered structure might be the centers of the initiation of renaturation, where the restoration of the initial native conformation of denatured proteins begins. Studies on the denaturation of proteins hardly clarify how the proteins fold into the native conformation during the successive residue-by-residue elongation of the polypeptide chain on the ribosome. | en_US |
| dc.identifier.citation | Basharov Mahmud A. Residual ordered structure in denatured proteins and the problem of protein folding. Indian Journal of Biochemistry & Biophysics. 2012 Feb; 49(1): 7-17. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/140213 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://nopr.niscair.res.in/handle/123456789/13586 | en_US |
| dc.subject | Protein Folding | en_US |
| dc.subject | Protein Residual Structure | en_US |
| dc.subject | Protein Denaturation | en_US |
| dc.subject.mesh | Elasticity | |
| dc.subject.mesh | Protein Conformation | |
| dc.subject.mesh | Protein Denaturation | |
| dc.subject.mesh | Protein Folding | |
| dc.subject.mesh | Proteins --chemistry | |
| dc.title | Residual ordered structure in denatured proteins and the problem of protein folding. | en_US |
| dc.type | Article | en_US |