Interaction of mithramycin with chromatin.

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dc.contributor.authorMir, M Aen_US
dc.contributor.authorDasgupta, Den_US
dc.date.accessioned2001-02-21en_US
dc.date.accessioned2009-05-27T09:05:59Z
dc.date.available2001-02-21en_US
dc.date.available2009-05-27T09:05:59Z
dc.date.issued2001-02-21en_US
dc.description.abstractMithramycin (MTR) is an anti-cancer antibiotic that blocks the macromolecular biosynthesis via reversible interaction with DNA template in the presence of bivalent metal ion such as Mg2+. In absence of DNA, mithramycin forms two types of complexes with Mg2+, complex I (with 1:1 stoichiometry in terms of MTR: Mg2+) and complex II (with 1:2 stoichiometry in terms of MTR: Mg2+). In an eukaryotic system, the drug would interact with chromatin, a protein-DNA complex. We have employed the spectroscopic techniques such as absorption and fluorescence to study the interaction of MTR: Mg2+ complexes with rat liver chromatin. In this report, we have shown that the two types of ligands have different binding potentials with the same chromatin. This supports our proposition that complexes I and II, are different molecular species. We have also shown that the histone protein(s) reduce the binding potential and the number of available sites for both ligands.en_US
dc.description.affiliationBiophysics Division, Saha Institute of Nuclear Physics, 37, Belgachia Road, Calcutta 700 037, W. Bengal, India.en_US
dc.identifier.citationMir MA, Dasgupta D. Interaction of mithramycin with chromatin. Indian Journal of Biochemistry & Biophysics. 2001 Feb-Apr; 38(1-2): 71-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26445
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChromatin --chemistryen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshHistones --chemistryen_US
dc.subject.meshKineticsen_US
dc.subject.meshLigandsen_US
dc.subject.meshLiver --metabolismen_US
dc.subject.meshMagnesium --metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshNucleic Acid Synthesis Inhibitors --chemistryen_US
dc.subject.meshPlicamycin --chemistryen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshRatsen_US
dc.subject.meshSpectrometry, Fluorescenceen_US
dc.titleInteraction of mithramycin with chromatin.en_US
dc.typeJournal Articleen_US
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