Evaluation of Effectiveness of Different Regimens of Centrifugation for a Single-spin Method of Pure Platelet-containing Plasma Preparation

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dc.contributor.authorPetrushenko, Viktoriaen_US
dc.contributor.authorGrebeniuk, Dmytroen_US
dc.contributor.authorNazarchuk, Halynaen_US
dc.date.accessioned2023-08-25T07:35:56Z
dc.date.available2023-08-25T07:35:56Z
dc.date.issued2022-08
dc.description.abstractAims: The aim of the study is to determine the optimal parameters of single centrifugation for the preparation of platelet-containing plasma (PCP) with maximum reduction of other cellular elements of the blood.Materials and Methods:30 conditionally healthy persons aged 18 to 60 years (36.9±11.2 years) were included in the study. A total of 12 centrifugation modes were studied: 110g×5min, 110g×10min, 110g×15min, 140g×5min, 140g×10min, 140g×15min, 160g×5min, 160g×10min, 160g×15min, 190g×5min, 190g×10min, 190g×15min. To evaluate the effectiveness of different centrifugation modes, in addition to the number of basic cellular elements, such indicators as platelet capture efficiency, platelet enrichment factor, erythrocyte-reducing efficiencies and leukocyte-reducing efficiencies were studied.Results:When examining the volumes of the obtained plasma containing platelets, it was found that almost all centrifugation modes allow obtaining significantly different volumes of the investigated blood fraction from the others (p<0.001). For clarity, the regimens were sorted according to the volume of platelet-containing plasma obtained, from the smallest to the largest. There was a progressive decrease in the numerical values of the concentration of platelets, erythrocytes and leukocytes in plasma samples. Also there was a progressive decrease in the numerical values of the coefficient of platelet enrichment.Conclusions:With a singlecentrifugation for the preparation of plasma containing platelets, the most effective mode is 160g × 10 min, which allows achieving a platelet enrichment factor of about 1.71 at a platelet concentration of 483.6 ± 45.4 × 109/l, a platelet capture efficiency of 85, 7 ± 0.1% and reductions of erythrocytes and leukocytes 98.76 ± 0.09% and 98.46 ± 0.14%, respectively.en_US
dc.identifier.affiliationsNational Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.en_US
dc.identifier.citationPetrushenko Viktoria, Grebeniuk Dmytro, Nazarchuk Halyna. Evaluation of Effectiveness of Different Regimens of Centrifugation for a Single-spin Method of Pure Platelet-containing Plasma Preparation. International Blood Research & Reviews. 2022 Jan; 13(4): 73-83en_US
dc.identifier.issn2321–7219
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/225658
dc.languageenen_US
dc.publisherSciencedomain Internationalen_US
dc.relation.issuenumber4en_US
dc.relation.volume13en_US
dc.source.urihttps://dx.doi.org/10.9734/IBRR/2022/v13i430188en_US
dc.subjectPlatelet-containing plasmaen_US
dc.subjectcentrifugationen_US
dc.subjectsingle-spin methoden_US
dc.subjectplatelet-rich plasma.en_US
dc.titleEvaluation of Effectiveness of Different Regimens of Centrifugation for a Single-spin Method of Pure Platelet-containing Plasma Preparationen_US
dc.typeJournal Articleen_US
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