Rt-PCR method for diagnosis and follow-up of hematological malignancies: first approach in Bangladesh.

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dc.contributor.authorSultana, Tanvira Afrozeen_US
dc.contributor.authorAbdul Mottalib, Mden_US
dc.contributor.authorIslam, Sirazulen_US
dc.contributor.authorKhan, Mohiuddin Ahmeden_US
dc.contributor.authorChoudhury, Subhagataen_US
dc.date.accessioned2008-04-12en_US
dc.date.accessioned2009-05-27T03:47:13Z
dc.date.available2008-04-12en_US
dc.date.available2009-05-27T03:47:13Z
dc.date.issued2008-04-12en_US
dc.descriptionBangladesh Medical Research Council Bulletin.en_US
dc.description.abstractNested reverse-transcriptase polymerase chain reaction (rt-PCR) was performed on 58 leukemia patients at BIRDEM Laboratory, as a pioneering work in Bangladesh. Thirty of themwere examined for the presence of BCR-ABL being clinically and morphologically diagnosed as chronic myeloid leukemia (CML) and 28 for PML-RARalpha fusion transcripts being clinically and morphologically diagnosed as acute promyelocytic leukemia (APL/ AML M3). The cases were selected for targeted therapy with imatinib mesylate and all-Trans retinoic acid (ATRA) to treat CML and APL respectively. Samples were received either before commencement or during therapy. In the positive cases, amplified DNA products were visible after gel electrophoresis and were reported accordingly. In case of BCR-ABL, positive results were found for five out of six (83.33%) untreated cases and 11 out of 24 (45.83%) treated cases. Positive results for PML-RARalpha were found for 12 out of 14 (85.70%) untreated cases and 11 out of 16 (68.75%) treated cases. A strong positive correlation was found between duration of treatment and negativity of PCR results in both the cases. In present times, the detection of minimal residual disease in patients undergoing treatment for hematological malignancies has become an important goal, not only to monitor the effectiveness of therapy but also to detect an impending relapse. This is the first time in Bangladesh that rt-PCR method is being employed to detect or monitor the presence of abnormal fusion genes in hematological malignancies.en_US
dc.description.affiliationLaboratory Department, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Shahbag, Dhaka. tanvira_sultana@yahoo.comen_US
dc.identifier.citationSultana TA, Abdul Mottalib M, Islam S, Khan MA, Choudhury S. Rt-PCR method for diagnosis and follow-up of hematological malignancies: first approach in Bangladesh. Bangladesh Medical Research Council Bulletin. 2008 Apr; 34(1): 1-11en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/369
dc.language.isoengen_US
dc.source.urihttps://www.banglajol.info/index.php/BMRCB/indexen_US
dc.source.urihttps://www.banglajol.info/index.php/BMRCB/article/view/1162/1176en_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAntineoplastic Agents --therapeutic useen_US
dc.subject.meshBangladeshen_US
dc.subject.meshChilden_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukemia, Myelogenous, Chronic, BCR-ABL Positive --diagnosisen_US
dc.subject.meshLeukemia, Promyelocytic, Acute --diagnosisen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPiperazines --therapeutic useen_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshPyrimidines --therapeutic useen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction --instrumentationen_US
dc.subject.meshTreatment Outcomeen_US
dc.subject.meshTretinoin --therapeutic useen_US
dc.titleRt-PCR method for diagnosis and follow-up of hematological malignancies: first approach in Bangladesh.en_US
dc.typeJournal Articleen_US
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