CpG DNA, liposome and refined antigen oral cholera vaccine.

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dc.contributor.authorLeelawongtawon, Ratreeen_US
dc.contributor.authorSomroop, Srinuanen_US
dc.contributor.authorChaisri, Uraien_US
dc.contributor.authorTongtawe, Pongsrien_US
dc.contributor.authorChongsa-nguan, Manasen_US
dc.contributor.authorKalambaheti, Thareeraten_US
dc.contributor.authorTapchaisri, Pramuanen_US
dc.contributor.authorPichyangkul, Sathiten_US
dc.contributor.authorSakolvaree, Yuwapornen_US
dc.contributor.authorKurazono, Hisaoen_US
dc.contributor.authorHayashi, Hideoen_US
dc.contributor.authorChaicumpa, Wanpenen_US
dc.date.accessioned2009-05-27T17:05:50Z
dc.date.available2009-05-27T17:05:50Z
dc.date.issued2003-12-17en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractAn oral cholera vaccine made up of three Vibrio cholerae antigens, i.e. lipopolysaccharide (LPS), recombinant toxin co-regulated pili (rTcpA) and heat-treated cholera toxin (H-CT) has been developed in six different formulations. Eight-week-old Wistar rats were divided into nine groups and immunized as follows: the first group received the oral vaccine 1 consisting of the three antigens (LPS, rTcpA and H-CT) associated with a liposome (L) and bacterial CpG-DNA (ODN#1826). The rats of groups 2 and 3 received oral vaccines 2 and 3 consisting of the liposome-associated three antigens with and without non-bacterial CpG-DNA (ODN#1982), respectively. Rats of groups 4 received oral vaccine 4 consisting of the three antigens mixed with the ODN#1826, similar to vaccine 1, but without liposome. Rats of groups 5 and 6 received oral vaccines 5 and 6 consisting of the three antigens with and without ODN#1982, respectively, similar to vaccines 2 and 3, but without liposome. Rats of groups 7, 8 and 9 received oral placebos, namely liposomes (L), ODN#1826 (CpG), and vaccine diluent, i.e. 5% NaHCO3 solution, respectively. All vaccines were given in three doses at 14-day intervals. It was found that the combination of liposome and ODN#1826 in vaccine 1 evoked the highest immune response to V. cholerae antigen compared to other vaccine formulations and placebos, as measured by the appearance of antigen-specific antibody-producing cells in the intestinal lamina propria. The immunogenicity according to the magnitude of the immune response was: V1>V2=V3>V4>V5=V6>V7=V8=V9. The results of this study indicate that CpG-DNA and liposome are effective mucosal adjuvants for an oral cholera vaccine prepared from refined V. cholerae antigens and their combination seems to be synergistic. The potential role of liposome as a vaccine delivery vehicle has been confirmed.en_US
dc.description.affiliationDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationLeelawongtawon R, Somroop S, Chaisri U, Tongtawe P, Chongsa-nguan M, Kalambaheti T, Tapchaisri P, Pichyangkul S, Sakolvaree Y, Kurazono H, Hayashi H, Chaicumpa W. CpG DNA, liposome and refined antigen oral cholera vaccine. Asian Pacific Journal of Allergy and Immunology. 2003 Dec; 21(4): 231-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36444
dc.language.isoengen_US
dc.subject.meshAdjuvants, Immunologicen_US
dc.subject.meshAdministration, Oralen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterial --biosynthesisen_US
dc.subject.meshAntigens, Bacterial --administration & dosageen_US
dc.subject.meshCholera --prevention & controlen_US
dc.subject.meshCholera Vaccines --administration & dosageen_US
dc.subject.meshCpG Islands --geneticsen_US
dc.subject.meshDNA --administration & dosageen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunity, Mucosalen_US
dc.subject.meshImmunizationen_US
dc.subject.meshLiposomes --administration & dosageen_US
dc.subject.meshMaleen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshVibrio cholerae --immunologyen_US
dc.titleCpG DNA, liposome and refined antigen oral cholera vaccine.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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