A rapid method for preparation of sarcolemma from frog skeletal muscle.
| dc.contributor.author | Zaidi, S I M | |
| dc.contributor.author | Pandey, R N | |
| dc.contributor.author | Kidwai, A M | |
| dc.contributor.author | Murti, C R Krishna | |
| dc.date.accessioned | 2015-07-20T10:55:50Z | |
| dc.date.available | 2015-07-20T10:55:50Z | |
| dc.date.issued | 1981-09 | |
| dc.description.abstract | A rapid method for the preparation of sarcolema from frog skeletal muscle has been described. The purified cell segments were transparent and devoid of contractile material. The Na+, Κ+ -ATPase and 5'-nucleotidase activities in sarcolemma purified by this method were comparable to those reported for sarcolemmal preparations purified by density gradient centrifugation. The preparation also possessed acid phosphatase, alkaline phosphatase and Κ+-activated, ouabain-sensitive p-nitrophenyl phosphatase activities. The cholesterol to phospholipid ratio of the sarcolemma was 0.33, indicating its high purity; further, the preparation was free from mitochondria and contractile proteins. | en_US |
| dc.identifier.citation | Zaidi S I M, Pandey R N, Kidwai A M, Murti C R Krishna. A rapid method for preparation of sarcolemma from frog skeletal muscle. Journal of Biosciences. 1981 Sept; 3(3): 293-302. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/160160 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ias.ac.in/jarch/jbiosci/3/293-302.pdf | en_US |
| dc.subject | Skeletal muscle | en_US |
| dc.subject | plasma membrane | en_US |
| dc.subject | sarcolemma | en_US |
| dc.title | A rapid method for preparation of sarcolemma from frog skeletal muscle. | en_US |
| dc.type | Article | en_US |