Sequential study of IgG antibody response in immunized rabbit and development of immunization protocol for raising monospecific antibody.

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dc.contributor.authorChande, Cen_US
dc.contributor.authorThakar, Y Sen_US
dc.contributor.authorPande, Sen_US
dc.contributor.authorDhanvijay, A Gen_US
dc.contributor.authorShrikhande, A Ven_US
dc.contributor.authorSaoji, A Men_US
dc.date.accessioned1996-01-01en_US
dc.date.accessioned2009-05-29T10:47:24Z
dc.date.available1996-01-01en_US
dc.date.available2009-05-29T10:47:24Z
dc.date.issued1996-01-01en_US
dc.description.abstractThe present study was planned to develop an immunization protocol to decide the bleeding modalities for harvesting anti IgG antibody from the immunized rabbit. A fourteen dose immunization protocol (four primary and ten boosters) of the purified human IgG spread over the one calendar year was executed. The antibody titre estimated by Reverse Single Radial Immunodiffusion displayed a six-phased pattern. The titre following the initial immunization ranged between nil to 1.02, characteristic of the primary response while the titres after 7th and 8th boosters (phase V) ranged between 0.5 to 3.87 consistent with secondary response. Phase II, III, IV had moderately elevated titres. The antibody titre amongst the six phases reached to its peak generally by the 12th day after the last dose of protocol and it took about 60 days to reach to its basal level. Administration of antigen with the higher levels of residual antibody did not produce high titre antibody and is probably ascribed to elimination of antigen through an immunecomplex mechanism. Based on the data we recommend that 15 batches. (3 per phase, phase I to phase V) with a total yield of 100 to 120 ml of serum can be procured from one immunised animal over the span of one calendar year and that should make the programme cost effective.en_US
dc.description.affiliationDepartment of Microbiology, Government Medical College, Nagpur.en_US
dc.identifier.citationChande C, Thakar YS, Pande S, Dhanvijay AG, Shrikhande AV, Saoji AM. Sequential study of IgG antibody response in immunized rabbit and development of immunization protocol for raising monospecific antibody. Indian Journal of Pathology & Microbiology. 1996 Jan; 39(1): 27-32en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/72814
dc.language.isoengen_US
dc.source.urihttps://www.ijpmonline.orgen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies --blooden_US
dc.subject.meshHumansen_US
dc.subject.meshImmunization Scheduleen_US
dc.subject.meshImmunoglobulin G --administration & dosageen_US
dc.subject.meshRabbitsen_US
dc.subject.meshTime Factorsen_US
dc.titleSequential study of IgG antibody response in immunized rabbit and development of immunization protocol for raising monospecific antibody.en_US
dc.typeJournal Articleen_US
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