Tissue Distribution Study of Apocynin By RP-HPLC With UV Detector

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dc.contributor.authorLiang, Zhiyanen_US
dc.contributor.authorLi, Huirouen_US
dc.contributor.authorWang, Kaiyuen_US
dc.contributor.authorOuyang, Jiabien_US
dc.contributor.authorYang, Mohuien_US
dc.contributor.authorLu, Qiweien_US
dc.contributor.authorXiang, Menghuaen_US
dc.contributor.authorYou, Jiayien_US
dc.contributor.authorJiang, Jieen_US
dc.contributor.authorLi, Shaen_US
dc.date.accessioned2020-11-18T10:29:10Z
dc.date.available2020-11-18T10:29:10Z
dc.date.issued2019-05
dc.description.abstractBackground Apocynin (4-hydroxy-3-methoxyacetophenone), an important active ingredient contained in root ofPicrorhiza kurroa Royle, has been widely investigated as an antioxidative and anti-inflammatory agent in kinds ofdisease models, and exerted certain efficacy deserving further research. However, little was known about thedisposal process of apocynin in vivo, which is important information required in drug research and development.Aim In this work, a tissue distribution study of apocynin was performed in Sprague-Dawley (SD) male rats to helpfurther understanding well the disposition of apocynin in vivo.Methods A simple HPLC-UV method was developed for measurement of apocynin concentration in rat tissues. Amixture of water-methanol (47:53, v/v) was used as solvent system, the flow rate was 1 mL/min, and the detectedwavelength was 279 nm. The method was validated and applied to the tissue distribution study of a single bolusintravenous administration of apocynin in SD male rats.Results The developed HPLC-UV method showed good specificity, precision, accuracy and extraction recovery. Thegood linearity was achieved within 0.8-32 μg/mL in tissues including heart, liver, spleen, lung, kidney and brain. Thelower limit of quantification (LLOQ) was 0.8 μg/mL. The method was well used in the study of tissue distribution ofapocynin. The results demonstrated that apocynin was distributed fast into the tested tissues and reached peakconcentration at 5 min after injection. Apocynin was mainly distributed in liver, kidney and lung within 15 minutesafter administration, and eliminated from the tissues with no sample be detected more than 2 h after doseen_US
dc.identifier.affiliationsCollege of Pharmacy, Jinan University, Guangzhou 510632, Chinaen_US
dc.identifier.affiliationsDispensary of Traditional Chinese Medicine, Hainan Provincial Hospital of Traditional Chinese Medicine, Haikou 570203, Chinaen_US
dc.identifier.affiliationsDongguan Institute of Jinan University, Dongguan 523808, Chinaen_US
dc.identifier.citationLiang Zhiyan, Li Huirou, Wang Kaiyu, Ouyang Jiabi, Yang Mohui, Lu Qiwei, Xiang Menghua, You Jiayi, Jiang Jie, Li Sha. Tissue Distribution Study of Apocynin By RP-HPLC With UV Detector. Journal of Pharmaceutical and Biomedical Sciences. 2019 May; 9(5): 48-56en_US
dc.identifier.issn2230-7885
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215709
dc.languageenen_US
dc.publisherLawarence Press Pvt. Ltd.en_US
dc.relation.issuenumber5en_US
dc.relation.volume9en_US
dc.source.urihttps://lawarencepress.com/ojs/index.php/JPBMS/article/view/561/pdf_279en_US
dc.subjectApocyninen_US
dc.subjectHPLCen_US
dc.subjecttissue distribution.en_US
dc.titleTissue Distribution Study of Apocynin By RP-HPLC With UV Detectoren_US
dc.typeJournal Articleen_US
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