Effect of Brine Concentration on Bacteria Isolated from Leafy Vegetables (Talinum triangulare, Telfairia occidentalis and Vernonia amygdalina)

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dc.contributor.authorIhechu , S. Cen_US
dc.contributor.authorRobinson , V. Ken_US
dc.contributor.authorAkani , N. P.en_US
dc.date.accessioned2023-06-27T06:26:59Z
dc.date.available2023-06-27T06:26:59Z
dc.date.issued2023-05
dc.description.abstractThe effect of brine on bacteria isolated from leafy vegetables was evaluated. Fresh waterleaf (Talinum triangulare), Pumpkin leaves (Telfairia occidentalis) and Bitter leaves (Vernonia amygdalina) including cooking salt were bought from vendors in Mile 3 market, Port Harcourt, Rivers State. Brine concentrations of 1, 2, 3, 4 and 5% were prepared by dissolving appropriate grams of salt in distilled water. Nine millilitres of the respective concentrations were transferred into clean test tubes, labelled, stoppered with foil and autoclaved at 121? for 15 minutes at 15psi. Sterile distilled water served as a control. The test isolates were standardized based on 0.5McFarland and 1mL each was introduced into different brine concentrations. The standard plate count was used to monitor brine effects on isolates and this was done hourly for six hours. Inoculated plates were incubated at 37? for 24 hours. After incubation, enumerated colonies were used to deduce isolate mortality. The total heterotrophic bacterial (THB) load of bitter, Pumpkin and water leaves were 3.49±0.4×106, 3.25±0.4×106 and 1.99±0.2×106 CFU/g, respectively. The staphylococcal counts for bitter, Pumpkin and water leaves were 1.65±0.3×104, 3.13±0.5×104 and 1.55±0.4×104 CFU/g, respectively. Total coliform counts for bitter, Pumpkin and water leaves were 1.52±0.8×105, 2.85±0.1×105 and 1.75±0.6×105 CFU/g, respectively. Staphylococcal counts of pumpkin leaves were significantly (P?0.05) higher than those obtained for bitter leaf and water leaf. There was no significant difference(P>0.05) in the THB and Coliform counts of all samples. E. coli was predominant in Pumpkin and water leaves while Staphylococcus sp was predominant in bitter leaves. The LC50 values for E. coli, Klebsiella, Staphylococcus, and Bacillus sp were; 5.39, 3.88, 1.62, and -0.41mg/ml, respectively. The LC50 showed that the brine was very lethal on Bacillus sp and Staphylococcus sp. High brine concentration is recommended to achieve reduced bacterial load.en_US
dc.identifier.affiliationsDepartment of Microbiology, Rivers State University, Nigeria.en_US
dc.identifier.citationIhechu S. C, Robinson V. K, Akani N. P.. Effect of Brine Concentration on Bacteria Isolated from Leafy Vegetables (Talinum triangulare, Telfairia occidentalis and Vernonia amygdalina). Microbiology Research Journal International. 2023 May; 33(3): 61-71en_US
dc.identifier.issn2456-7043
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/217202
dc.languageenen_US
dc.publisherSciencedomain Internationalen_US
dc.relation.issuenumber3en_US
dc.relation.volume33en_US
dc.source.urihttps://doi.org/10.9734/mrji/2023/v33i31372en_US
dc.subjectBrineen_US
dc.subjectleafy vegetablesen_US
dc.subjectbacterial isolatesen_US
dc.titleEffect of Brine Concentration on Bacteria Isolated from Leafy Vegetables (Talinum triangulare, Telfairia occidentalis and Vernonia amygdalina)en_US
dc.typeJournal Articleen_US
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