Genetic Diversity and Molecular Surveillance of Antimalarial Drug Resistance of Plasmodium falciparum among Hospitals Patients in Benue State Nigeria

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dc.contributor.authorAdulugba, O. Aen_US
dc.contributor.authorAmali, Oen_US
dc.contributor.authorManyi, M. Men_US
dc.contributor.authorIkpa, T. Fen_US
dc.contributor.authorObisike, V. U.en_US
dc.date.accessioned2023-06-27T06:26:08Z
dc.date.available2023-06-27T06:26:08Z
dc.date.issued2022-01
dc.description.abstractIntroduction: Malaria is a febrile illness caused by parasites of the genus Plasmodium and transmitted by female Anopheles mosquitoes. The genetic diversity and antimalarial drug resistance of Plasmodium falciparum are some of the major challenges of malaria control programme in Nigeria. Aim: This study was aimed at determining the genetic diversity, and molecular surveillance of antimalarial drug resistance among patients attending Government hospitals in Benue State, Nigeria. Methodology: Plasmodium falciparum deoxyribonucleic acid was extracted from dried blood spots of 60 positive malariacases among the patients. The diversity of Plasmodium falciparum was done by genotyping 3D7 and FC27 families of merozoite surface protein- 2 alleles. The Plasmodium falciparum multidrug resistance 1 and Plasmodium falciparum kelch13 genes of Plasmodium falciparum were also amplified and assessed by restriction fragment length polymorphism (RFLP) to survey molecular resistance to antimalarial drugs. Results: The results showed that the frequency of 3D7 allele 37(61.7%) was higher than FC27 allele 18(30.0%). The frequency of merozoite surface protein- 2 infections with both allelic types was 5(8.3%). There was a significant difference in the distribution of the merozoite surface protein two alleles (?2=25.9,df=2 P<.0.001). Both the Plasmodium falciparum multidrug resistance 1 Asparagine 86Tyrosine (N86Y) and Aspartic acid 1246Tyrosine (D1246Y), had 100 % mutant while the 100% while the Plasmodium falciparum kelch13 G449A had 100% wild type allele. Conclusion: The current study underscores the need for frequent monitoring of indicators of antimalaria drug resistance in Nigeria.en_US
dc.identifier.affiliationsDepartment of Zoology, Federal University of Agriculture, Makurdi, Benue State, Nigeriaen_US
dc.identifier.affiliationsDepartment of Zoology, Federal University of Agriculture, Makurdi, Benue State, Nigeriaen_US
dc.identifier.affiliationsDepartment of Zoology, Federal University of Agriculture, Makurdi, Benue State, Nigeriaen_US
dc.identifier.affiliationsDepartment of Zoology, Federal University of Agriculture, Makurdi, Benue State, Nigeriaen_US
dc.identifier.affiliationsDepartment of Animal and Environmental Biology, Abia State University, Uturu, Abia State, Nigeria.en_US
dc.identifier.citationAdulugba O. A, Amali O, Manyi M. M, Ikpa T. F, Obisike V. U.. Genetic Diversity and Molecular Surveillance of Antimalarial Drug Resistance of Plasmodium falciparum among Hospitals Patients in Benue State Nigeria. Sciencedomain International. 2022 Jan; 32(1): 1-10en_US
dc.identifier.issn2456-7043
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/217151
dc.languageenen_US
dc.publisherMicrobiology Research Journal Internationalen_US
dc.relation.issuenumber1en_US
dc.relation.volume32en_US
dc.source.urihttps://doi.org/10.9734/mrji/2022/v32i130360en_US
dc.subjectGenetic variationsen_US
dc.subjectinvestigationsen_US
dc.subjectdrug efficacyen_US
dc.subjectmalaria parasiteen_US
dc.titleGenetic Diversity and Molecular Surveillance of Antimalarial Drug Resistance of Plasmodium falciparum among Hospitals Patients in Benue State Nigeriaen_US
dc.typeJournal Articleen_US
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