Serum protein profiling to identify biomarkers for small renal cell carcinoma.

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dc.contributor.authorXu, Gang
dc.contributor.authorHou, Chen-Rui
dc.contributor.authorJiang, Hao-Wen
dc.contributor.authorXiang, Cui-Qin
dc.contributor.authorShi, Nong
dc.contributor.authorYuan, Hai-Chuan
dc.contributor.authorDing, Qiang
dc.contributor.authorZhang, Yuan-Fang
dc.date.accessioned2011-11-14T08:56:57Z
dc.date.available2011-11-14T08:56:57Z
dc.date.issued2010-08
dc.description.abstractDiagnostic biomarkers for early detection of renal cell carcinoma (RCC) are in great need. In the present study, we compared the serum protein profiles of patients with small RCC to those of healthy individuals to identify the differentially expressed proteins with potential to serve as biomarkers. Serum samples were collected from 10 patients with small RCC and 10 healthy individuals. The serum protein expression profiles were analyzed by two-dimensional (2-D) gel electrophoresis. Twenty-seven proteins with differences in expression levels between RCC patients and healthy volunteers were identified. Of these, 19 were expressed at different levels and eight were expressed in serum from the RCC group, but not from the control group. Six differentially expressed proteins identified by using mass spectrometry included coagulation factor XIII B, complement C3 and its precursor, misato homolog 1 (isoform CRA_b), hemopexin, and alpha-1-B-glycoprotein. Some of these serum proteins are known regulators of tumor progression in human malignancies. In conclusion, we successfully applied 2-D gel electrophoresis and identified six serum proteins differentially expressed between patients with small RCC and healthy volunteers. These proteins may provide novel biomarkers for early detection and diagnosis of human RCC.en_US
dc.identifier.citationXu Gang, Hou Chen-Rui, Jiang Hao-Wen, Xiang Cui-Qin, Shi Nong, Yuan Hai-Chuan, Ding Qiang, Zhang Yuan-Fang. Serum protein profiling to identify biomarkers for small renal cell carcinoma. Indian Journal of Biochemistry & Biophysics. 2010 Aug; 47(4): 211-218.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135268
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/10120/1/IJBB%2047%284%29%20211-218.pdfen_US
dc.subjectRenal cell carcinomaen_US
dc.subjectSerum biomarkersen_US
dc.subjectTwo-dimensional gel electrophoresisen_US
dc.subject.meshAged
dc.subject.meshBlood Proteins --chemistry
dc.subject.meshCarcinoma, Renal Cell --blood
dc.subject.meshCarcinoma, Renal Cell --metabolism
dc.subject.meshCase-Control Studies
dc.subject.meshElectrophoresis, Gel, Two-Dimensional
dc.subject.meshFemale
dc.subject.meshGene Expression Regulation, Neoplastic
dc.subject.meshHumans
dc.subject.meshKidney Neoplasms --blood
dc.subject.meshKidney Neoplasms --metabolism
dc.subject.meshMale
dc.subject.meshMass Spectrometry --methods
dc.subject.meshMiddle Aged
dc.subject.meshProtein Isoforms
dc.subject.meshTrypsin --chemistry
dc.subject.meshTumor Markers, Biological --metabolism
dc.titleSerum protein profiling to identify biomarkers for small renal cell carcinoma.en_US
dc.typeArticleen_US
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