Antigenic differences between axenic amastigotes & promastigotes of Leishmania donovani.

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dc.contributor.authorChattopadhayay, Ren_US
dc.contributor.authorKaur, Sen_US
dc.contributor.authorGanguly, N Ken_US
dc.contributor.authorMahajan, R Cen_US
dc.date.accessioned1996-12-01en_US
dc.date.accessioned2009-05-27T06:54:14Z
dc.date.available1996-12-01en_US
dc.date.available2009-05-27T06:54:14Z
dc.date.issued1996-12-01en_US
dc.description.abstractThe axenic amastigotes of an Indian strain of L. donovani have been generated from the promastigote form at 37 degrees C in RPMI-1640 medium, pH 6.0 +/- 0.5, supplemented with 10 per cent heat inactivated foetal calf serum and these are being maintained through serial subculturing under the same conditions. The present study was carried out to differentiate axenic amastigote from the promastigote stage on the basis of their antigenic constitution and also to look for any immunoreactive antigen(s) specific to axenic amastigotes. SDS-PAGE analysis revealed a few stage specific and some conserved antigenic fractions in both the stages. On immunoblotting with immune sera raised against the membrane fractions of the axenic amastigotes, the 200 kDa antigenic fraction of axenic amastigote was found to be highly reactive. When the immune sera raised against the membranes of both stages were checked by immunofluorescence no cross reactivity was observed at higher dilutions. These findings showed that there are some antigenic diversities as well as similarities between the two stages of L. donovani cultured in vitro. Also, the 200 kD fraction of axenic amastigote appeared to be an immunodominant antigen specific to that stage.en_US
dc.description.affiliationDepartment of Parasitology, Postgraduate Institute of Medical Education & Research, Chandigarh.en_US
dc.identifier.citationChattopadhayay R, Kaur S, Ganguly NK, Mahajan RC. Antigenic differences between axenic amastigotes & promastigotes of Leishmania donovani. Indian Journal of Medical Research. 1996 Dec; 104(): 349-54en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/18437
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Protozoan --analysisen_US
dc.subject.meshEpitopesen_US
dc.subject.meshGerm-Free Lifeen_US
dc.subject.meshLeishmania donovani --growth & developmenten_US
dc.titleAntigenic differences between axenic amastigotes & promastigotes of Leishmania donovani.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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