Purification and characterization of a novel zinc chelating peptides from Holothuria scabra and its ex vivo absorption activity in the small intestine

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dc.contributor.authorSyahputra, Gen_US
dc.contributor.authorSandhiutami, NMDen_US
dc.contributor.authorHariyatun, Hen_US
dc.contributor.authorHapsari, Yen_US
dc.contributor.authorGustini, Nen_US
dc.contributor.authorSari, Men_US
dc.contributor.authorSiwi, OGRen_US
dc.contributor.authorPutra, MYen_US
dc.contributor.authorFadilah, Fen_US
dc.contributor.authorLouisa, M.en_US
dc.date.accessioned2024-11-30T11:21:20Z
dc.date.available2024-11-30T11:21:20Z
dc.date.issued2024-09
dc.description.abstractZinc supplements currently marketed in Indonesia are second-generation zinc supplements, which must be improved for the effectiveness caused by their low absorption rate and side effects on the gastrointestinal tract. Third- generation zinc supplements are currently being investigated to improve the effectiveness of the previous generation of supplements. The present study aimed to discover a new alternative source of zinc-chelating peptide (ZCP) from Holothuria scabra. In addition, the research investigates the ZCP characteristics and absorption abilities in an ex vivo evaluation using the everted gut sac model method. The results obtained were six zinc peptides: Asp-Asp-Ala- Phe-Gln-Ala-Phe-Cys; Thr-Asp-Asn-Leu; Leu-Gly-Cys; Pro-Gly-Thr; Ser-Cys; and Pro-Tyr. The characterization showed that the resulting peptides comprised two to eight amino acids with molecular weights between 208 and 915 Da. The highest zinc binding capacity of the peptides was 92.11 mg/100 g at 30 kDa for molecular weight. Fourier- transform infrared spectra indicate that zinc ions (Zn 2+ ) bind to carboxyl groups and interact with nitrogen atoms in amino acids and amides. The scanning electron microscope-energy dispersion spectroscopy results stated that, topographically and morphologically, ZCP has a different surface with peptides with Zn composition in the sample. Furthermore, we evaluated the peptide fractions for ex vivo absorption in the small intestine using the everted gut sac method. We found that zinc-binding peptides transported and enhanced zinc absorption in the duodenum segment at 60 minutes, resulting in a 40% increase in absorption rate. The potency of ZCPs from H. scabra as an alternative to third-generation zinc supplements with higher absorption than previous-generation supplements.en_US
dc.identifier.affiliationsDoctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Research Center for Vaccine and Drug, National Research and Innovation Agency, Cibinong, Indonesiaen_US
dc.identifier.affiliationsDepartment of Pharmacy, Faculty of Pharmacy, University of Pancasila, Jakarta, Indonesiaen_US
dc.identifier.affiliationsResearch Center for Genetic Engineering, National Research and Innovation Agency, Cibinong, Indonesiaen_US
dc.identifier.affiliationsResearch Center for Vaccine and Drug, National Research and Innovation Agency, Cibinong, Indonesiaen_US
dc.identifier.affiliationsResearch Center for Vaccine and Drug, National Research and Innovation Agency, Cibinong, Indonesiaen_US
dc.identifier.affiliationsResearch Center for Vaccine and Drug, National Research and Innovation Agency, Cibinong, Indonesiaen_US
dc.identifier.affiliationsResearch Center for Vaccine and Drug, National Research and Innovation Agency, Cibinong, Indonesiaen_US
dc.identifier.affiliationsResearch Center for Vaccine and Drug, National Research and Innovation Agency, Cibinong, Indonesiaen_US
dc.identifier.affiliationsDepartment of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesiaen_US
dc.identifier.affiliationsDepartment of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesiaen_US
dc.identifier.citationSyahputra G, Sandhiutami NMD, Hariyatun H, Hapsari Y, Gustini N, Sari M, Siwi OGR, Putra MY, Fadilah F, Louisa M.. Purification and characterization of a novel zinc chelating peptides from Holothuria scabra and its ex vivo absorption activity in the small intestine. Journal of Applied Pharmaceutical Science. 2024 Sept; 14(9): 235-246en_US
dc.identifier.issn2231-3354
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/237703
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber9en_US
dc.relation.volume14en_US
dc.source.urihttps://doi.org/10.7324/JAPS.2024.180224en_US
dc.subjectHolothuria scabraen_US
dc.subjectintestinal simulation bioassayen_US
dc.subjectorganic zincen_US
dc.subjectpeptide hydrolysatesen_US
dc.subjectzinc absorption.en_US
dc.titlePurification and characterization of a novel zinc chelating peptides from Holothuria scabra and its ex vivo absorption activity in the small intestineen_US
dc.typeJournal Articleen_US
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