Development and evaluation of an in vitro isolation of street rabies virus in mouse neuroblastoma cells as compared to conventional tests used for diagnosis of rabies.

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dc.contributor.authorChhabra, Men_US
dc.contributor.authorMittal, Ven_US
dc.contributor.authorJaiswal, Ren_US
dc.contributor.authorMalik, Sen_US
dc.contributor.authorGupta, Men_US
dc.contributor.authorLal, Sen_US
dc.date.accessioned2007-07-29en_US
dc.date.accessioned2009-05-28T09:19:12Z
dc.date.available2007-07-29en_US
dc.date.available2009-05-28T09:19:12Z
dc.date.issued2007-07-29en_US
dc.description.abstractIn vitro isolation of rabies virus using mouse neuroblastoma cells (MNA) was evaluated. The sensitivity and reliability of in vitro procedure was performed in comparison with mouse inoculation test (MIT), the in vivo method of virus isolation, direct fluorescent antibody test (FAT) and Sellers staining. Of the 33 animal brain samples tested, 24 (72.72%) were positive by MIT. Sensitivity of Sellers stain, FAT and rapid tissue culture infection test (RTCIT) was found to be 54.16, 100 and 91.6% respectively. Concordance of Sellers stain, FAT, RTCIT with MIT was found to be 66.6, 100 and 93.93% respectively. Two samples which were positive by FAT and MIT showed gross contamination in cell lines, which is one of the drawbacks of RTCIT. However, rabies virus could be isolated in MNA cells from two of the eight human cerebrospinal fluid (CSF) samples from clinico-epidemiologically suspected cases of rabies. Both MIT and FAT showed negative results in the two CSF samples. RTCIT appears to be a fast and reliable alternative to MIT and holds promise in antemortem diagnosis of rabies, which is otherwise, a challenging task for a reference laboratory.en_US
dc.description.affiliationWHO Collaborating Centre for Rabies Epidemiology National Institute of Communicable Diseases, New Delhi - 110 054, India. malachhabra@yahoo.co.inen_US
dc.identifier.citationChhabra M, Mittal V, Jaiswal R, Malik S, Gupta M, Lal S. Development and evaluation of an in vitro isolation of street rabies virus in mouse neuroblastoma cells as compared to conventional tests used for diagnosis of rabies. Indian Journal of Medical Microbiology. 2007 Jul; 25(3): 263-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/53751
dc.language.isoengen_US
dc.source.urihttps://www.ijmm.orgen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain --virologyen_US
dc.subject.meshCatsen_US
dc.subject.meshCattleen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshDogsen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshNeuroblastoma --pathologyen_US
dc.subject.meshRabies --cerebrospinal fluiden_US
dc.subject.meshRabies virus --isolation & purificationen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshVirology --methodsen_US
dc.titleDevelopment and evaluation of an in vitro isolation of street rabies virus in mouse neuroblastoma cells as compared to conventional tests used for diagnosis of rabies.en_US
dc.typeComparative Studyen_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
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