Immunohistochemical study of the expression of human groEL-stress protein in human nervous tissue.

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dc.contributor.authorKhanna, Nen_US
dc.contributor.authorShankar, S Ken_US
dc.contributor.authorChandramuki, Aen_US
dc.contributor.authorJagannath, Cen_US
dc.date.accessioned1996-02-01en_US
dc.date.accessioned2009-05-27T07:47:45Z
dc.date.available1996-02-01en_US
dc.date.available2009-05-27T07:47:45Z
dc.date.issued1996-02-01en_US
dc.description.abstractMonoclonal antibody (ML-30) directed against 65 kDa stress protein of mycobacteria, is shown to identify human cellular protein homologous with the groEL heat shock protein in many prokaryotes. Immunohistochemical survey of nervous tissue, both central and peripheral, from patients dying of various inflammatory, degenerative and neoplastic conditions and from experimental animals, using this antibody showed punctate granular staining of the cells to a variable degree. The astrocytes showed strong immunolabelling. The normal neurons and oligodendroglia stained variably, while abnormal neurons were darkly labelled. Ependymal cells showed apical granular positivity. The ubiquitinated inclusion bodies in amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease were not recognised by the ML-30 antibody. In diseased and stressed nervous tissue from experimental animals, the expression of the ML-30 recognisable stress protein was variable. The epitope recognised by ML-30 was found stable in postmortem tissues collected up to 36 h after death and processed for paraffin sectioning, after fixation in formalin for many years. Enhanced expression of the human groEL stress protein homologue in mammalian nervous tissue following various forms of stress may play a role in modulating the extent of tissue damage by autoimmune mechanism because of its high immunogenic nature and constitutive presence in the cells.en_US
dc.description.affiliationDepartment of Neuromicrobiology, National Institute of Mental Health & Neurosciences, Bangalore.en_US
dc.identifier.citationKhanna N, Shankar SK, Chandramuki A, Jagannath C. Immunohistochemical study of the expression of human groEL-stress protein in human nervous tissue. Indian Journal of Medical Research. 1996 Feb; 103(): 103-11en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/22099
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain Chemistry --physiologyen_US
dc.subject.meshGerbillinaeen_US
dc.subject.meshGroEL Protein --analysisen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshNerve Tissue Proteins --analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshSaimirien_US
dc.subject.meshSpinal Cord --chemistryen_US
dc.titleImmunohistochemical study of the expression of human groEL-stress protein in human nervous tissue.en_US
dc.typeJournal Articleen_US
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