Fluorimetric probes of the individual and competitive binding of 1-anilinonaphthalene-8-sulfonate, eosine and fluorescene to bovine serum albumin.
| dc.contributor.author | Pal, M K | en_US |
| dc.contributor.author | Patra, S K | en_US |
| dc.date.accessioned | 1994-04-01 | en_US |
| dc.date.accessioned | 2009-05-27T09:42:39Z | |
| dc.date.available | 1994-04-01 | en_US |
| dc.date.available | 2009-05-27T09:42:39Z | |
| dc.date.issued | 1994-04-01 | en_US |
| dc.description.abstract | Fluorescence of 1-anilinonaphthalene-8-sulfonate (ANS) is greatly enhanced on its binding to bovine serum albumin (BSA). Fluorimetric titration shows that three ANS molecules bind per BSA molecule. The enhanced fluorescence of BSA-ANS is quenched by eosine (EOS); and one EOS physically displaces one ANS bound to BSA. The enhanced fluorescence of free ANS in the hydrophobic environment of the nonionic surfactant Triton X 100 is also quenched by EOS but by an energy transfer mechanism. The dye fluorescene (FLSN) also quenches the fluorescence of BSA-bound ANS, but by the energy transfer mechanism. The binding region of ANS in BSA has been speculated. | en_US |
| dc.description.affiliation | Department of Biochemistry & Biophysics, University of Kalyani, West Bengal, India. | en_US |
| dc.identifier.citation | Pal MK, Patra SK. Fluorimetric probes of the individual and competitive binding of 1-anilinonaphthalene-8-sulfonate, eosine and fluorescene to bovine serum albumin. Indian Journal of Biochemistry & Biophysics. 1994 Apr; 31(2): 109-14 | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/28329 | |
| dc.language.iso | eng | en_US |
| dc.source.uri | https://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.asp | en_US |
| dc.subject.mesh | Anilino Naphthalenesulfonates | en_US |
| dc.subject.mesh | Binding, Competitive | en_US |
| dc.subject.mesh | Eosine Yellowish-(YS) | en_US |
| dc.subject.mesh | Fluoresceins | en_US |
| dc.subject.mesh | Fluorescent Dyes | en_US |
| dc.subject.mesh | Kinetics | en_US |
| dc.subject.mesh | Serum Albumin, Bovine --chemistry | en_US |
| dc.subject.mesh | Spectrometry, Fluorescence --methods | en_US |
| dc.title | Fluorimetric probes of the individual and competitive binding of 1-anilinonaphthalene-8-sulfonate, eosine and fluorescene to bovine serum albumin. | en_US |
| dc.type | Comparative Study | en_US |
| dc.type | Journal Article | en_US |
| dc.type | Research Support, Non-U.S. Gov't | en_US |
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