A cysteine protease of Dieffenbachia maculata.

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dc.contributor.authorChitre, Aen_US
dc.contributor.authorPadmanabhan, Sen_US
dc.contributor.authorShastri, N Ven_US
dc.date.accessioned1998-12-21en_US
dc.date.accessioned2009-05-27T09:21:48Z
dc.date.available1998-12-21en_US
dc.date.available2009-05-27T09:21:48Z
dc.date.issued1998-12-21en_US
dc.description.abstractPlants of the genus Dieffenbachia, very popular as indoor ornamental plants, are known for their toxic as well as therapeutic properties. Their toxic manifestations have been partly attributed to their proteolytic activity. The work described in the present paper shows that stem leaves and petiole of Dieffenbachia maculata Schott, a commonly grown species, contain significant proteolytic activity, different parts showing different types of protease activities. Stem showed the highest enzyme activity and this protease was purified about 55 fold by solvent precipitation, gel filtration and ion exchange chromatography. The enzyme has a relative molecular mass of 61 kDa as determined by SDS-PAGE and has an optimum pH of 8.0 and optimum temperature of 50 degrees C. Effects of various substrates, inhibitors and activators indicate that the enzyme is a cysteine protease with leucylpeptidase activity.en_US
dc.description.affiliationUniversity Department of Biochemistry, Nagpur University, India.en_US
dc.identifier.citationChitre A, Padmanabhan S, Shastri NV. A cysteine protease of Dieffenbachia maculata. Indian Journal of Biochemistry & Biophysics. 1998 Dec; 35(6): 358-63en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27275
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAngiosperms --enzymologyen_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshCysteine Endopeptidases --chemistryen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshPlant Components --enzymologyen_US
dc.subject.meshTime Factorsen_US
dc.titleA cysteine protease of Dieffenbachia maculata.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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