Zymogram patterns of Naegleria spp isolated from natural water sources in Taling Chan district, Bangkok.

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dc.contributor.authorTiewcharoen, Supathraen_US
dc.contributor.authorKomalamisra, Narumonen_US
dc.contributor.authorJunnu, Virachen_US
dc.date.accessioned2009-05-27T15:48:07Z
dc.date.available2009-05-27T15:48:07Z
dc.date.issued2004-06-05en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractA genetic approach was cited for species detection of the ameba genus Naegleria using allozyme electrophoresis to characterize the trophozoite stage of three strains of Naegleria fowleri isolated from patients with primary amebic meningoencephalitis, five thermophilic (45 degrees C) Naegleria spp isolated from natural water sources in the Taling Chan district, and a reference control strain, Naegleria fowleri CDC VO 3081. Isoenzymes of ameba whole-cell extracts were analyzed by vertical polyacrylamide slab gel electrophoresis to determine whether there was any correlation between different strains of the ameba. The results showed that five out of fifteen enzymes; aldehyde oxidase (ALDOX), aldolase (ALD), a-glycerophosphate dehydrogenase (a-GPDH), xanthine dehydrogenase (XDH), and glutamate oxaloacetate transaminase (GOT), were undetectable in the pathogenic strains, while the other enzymes; esterase (EST), fumerase (FUM), glucose-6-phosphate dehydrogenase (G-6-PDH), glucose phosphate isomerase (GPI), isocitate dehydrogenase (IDH), lactate dehydrogenase (LDH), leucine aminopeptidase (LAP), malic enzyme (ME), glucose phosphomutase (GPM), and malate dehydrogenase (MDH), were detected. Naegleria fowleri strains were biochemically the most homogeneous. They showed intraspecific isoenzyme variation that allowed them to be grouped. In contrast, the allozyme patterns (EST 1-7, IDH) of Naegleria spp isolated from the environment showed interspecific isoenzyme variations from the pathogenic Naegleria strain. In conclusion, this study recognized the zymograms of the Naegleria fowleri strains were heterogenically different from the thermophilic 45 degrees C Naegleria spp isolated from the environment.en_US
dc.description.affiliationDepartment of Parasitology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand. supatth@Thaimail.comen_US
dc.identifier.citationTiewcharoen S, Komalamisra N, Junnu V. Zymogram patterns of Naegleria spp isolated from natural water sources in Taling Chan district, Bangkok. The Southeast Asian Journal of Tropical Medicine and Public Health. 2004 Jun; 35(2): 275-80en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/33693
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2004_35_2/08-3199.pdfen_US
dc.subject.meshAllelesen_US
dc.subject.meshAmebiasis --parasitologyen_US
dc.subject.meshAmoeba --enzymologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCentral Nervous System Protozoal Infections --parasitologyen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gel --methodsen_US
dc.subject.meshFresh Water --analysisen_US
dc.subject.meshGlucosephosphate Dehydrogenaseen_US
dc.subject.meshIsoenzymes --analysisen_US
dc.subject.meshNaegleria fowleri --classificationen_US
dc.subject.meshSwimmingen_US
dc.subject.meshThailanden_US
dc.titleZymogram patterns of Naegleria spp isolated from natural water sources in Taling Chan district, Bangkok.en_US
dc.typeJournal Articleen_US
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