Cholesteryl-(2'-hydroxy)-ethyl ether—A potential cholesterol substitute for studies in membranes.
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Date
1988-06
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Abstract
The yeast sterol auxotroph GL-7, which grows well on ergosterol and
cholesterol, was used to study the ability of cholesteryl-(2'–hydroxy)-ethyl ether to substitute
for cholesterol. In this compound the 3j3-hydroxyl group of cholesterol is replaced by
ethylene glycol and the resulting ether still retains the amphiphilic character of cholesterol.
Cholesteryl-(2Lhydroxy)-ethyl ether was found to support the growth of GL-7 as effectively
as cholesterol. Crystal violet permeability and membrane order parameter determined using
a spin label were similar for cells grown on these sterols. The ability of such ethylene glycol
derivatives to substitute for cholesterol in both artificial and natural membranes should
help in designing suitable spacers through which molecules can be linked to cholesterol
without affecting the normal function of cholesterol in membranes. This in turn should
prove useful in studies with surface -modified liposomes.
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Keywords
Cholesteryl-(2'hydroxy)-ethyl ether, yeast sterol auxotroph, membranes, anchor molecule
Citation
Lala Anil K, Kumari S Nanda. Cholesteryl-(2'-hydroxy)-ethyl ether—A potential cholesterol substitute for studies in membranes. Journal of Biosciences. 1988 Jun; 13(2): 147-151.