A comparative analysis of green fluorescent protein and beta-glucuronidase protein-encoding genes as a reporter system for studying the temporal expression profiles of promoters.

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dc.contributor.authorKavita, Pen_US
dc.contributor.authorBurma, Pradeep Kumaren_US
dc.date.accessioned2008-09-14en_US
dc.date.accessioned2009-06-01T14:48:29Z
dc.date.available2008-09-14en_US
dc.date.available2009-06-01T14:48:29Z
dc.date.issued2008-09-14en_US
dc.description.abstractThe assessment of activity of promoters has been greatly facilitated by the use of reporter genes. However, the activity as assessed by reporter gene is a reflection of not only promoter strength, but also that of the stability of the mRNA and the protein encoded by the reporter gene. While a stable reporter gene product is an advantage in analysing activities of weak promoters, it becomes a major limitation for understanding temporal expression patterns of a promoter, as the reporter product persists even after the activity of the promoter ceases. In the present study we undertook a comparative analysis of two reporter genes, beta-glucuronidase (gus) and green fluorescent protein (sgfp), for studying the temporal expression pattern of tapetum-specific promoters A9 (Arabidopsis thaliana) and TA29 (Nicotiana tabacum). The activity of A9 and TA29 promoters as assessed by transcript profiles of the reporter genes (gus or sgfp ) remained the same irrespective of the reporter gene used. However, while the deduced promoter activity using gus was extended temporally beyond the actual activity of the promoter, sgfp as recorded through its fluorescence correlated better with the transcription profile. Our results thus demonstrate that sgfp is a better reporter gene compared to gus for assessment of temporal activity of promoters. Although several earlier reports have commented on the possible errors in deducing temporal activities of promoters using GUS as a reporter protein, we experimentally demonstrate the advantage of using reporter genes such as gfp for analysis of temporal expression patterns.en_US
dc.description.affiliationDepartment of Genetics, University of Delhi South Campus, New Delhi, India.en_US
dc.identifier.citationKavita P, Burma PK. A comparative analysis of green fluorescent protein and beta-glucuronidase protein-encoding genes as a reporter system for studying the temporal expression profiles of promoters. Journal of Biosciences. 2008 Sep; 33(3): 337-43en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111091
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshArabidopsis --geneticsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGenes, Reporteren_US
dc.subject.meshGlucuronidase --geneticsen_US
dc.subject.meshGreen Fluorescent Proteins --geneticsen_US
dc.subject.meshMustard Plant --geneticsen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTobacco --geneticsen_US
dc.subject.meshTransformation, Geneticen_US
dc.titleA comparative analysis of green fluorescent protein and beta-glucuronidase protein-encoding genes as a reporter system for studying the temporal expression profiles of promoters.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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