Modulation of radiation-induced cytotoxicity in U 87 cells by RH-3 (a preparation of Hippophae rhamnoides).
dc.contributor.author | Agrawala, P K | |
dc.contributor.author | Adhikari, J S | |
dc.date.accessioned | 2011-12-16T06:39:51Z | |
dc.date.available | 2011-12-16T06:39:51Z | |
dc.date.issued | 2009-11 | |
dc.description.abstract | Background & objectives: Hippophae rhamnoides L. has been widely exploited for medicinal purposes and an extract of its whole berries coded as RH-3 has been found to render radioprotection. Effect of pre-irradiation treatment of up to 10 μg/ml RH-3 was studied in U 87 cells using MTT assay. This study aims at unraveling the mechanism of action of RH-3 in amelioration of radiation-induced cytotoxicity in vitro. Methods: Most effective doses selected were studied further for the elucidation of radiomodifying properties of RH-3, especially with respect to early and late events of apoptosis. Results: RH-3 at concentrations of 7.5 and 10 μg/ml (-15 min) were found most effective in protecting against 2 Gy induced cytotoxicity in terms of MTT reducing ability in U 87 cells. RH-3 was observed to mitigate radiation-induced cellular and mitochondrial free radicals. Mitochondrial membrane potential depletion (studied up to 12 h) was prevented by RH-3 pre-irradiation administration. It could also restore the level of antiapoptotic protein Bcl-2 at 24 and 48 h comparable to the control value. RH-3 also prevented radiation-induced increase in mitochondrial mass at 48 and 72 h post-treatment and the values were comparable to that of control cells. Annexin-V-FITC assay at 12 and 24 h time intervals indicated significant protection against radiation-induced apoptosis by RH-3 pre-irradiation treatment. Interpretation & conclusion: Our findings showed that probably RH-3 acts as an antioxidant preventing cellular and mitochondrial free radical generation that could contribute to its ability to inhibit radiationinduced apoptosis and cytotoxicity. | en_US |
dc.identifier.citation | Agrawala P K, Adhikari J S. Modulation of radiation-induced cytotoxicity in U 87 cells by RH-3 (a preparation of Hippophae rhamnoides). Indian Journal of Medical Research. 2009 Nov; 130(5): 542-549. | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/135935 | |
dc.language.iso | en | en_US |
dc.source.uri | https://icmr.nic.in/ijmr/2009/November/1108.pdf | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | flow cytometry | en_US |
dc.subject | free radicals | en_US |
dc.subject | Hippophae rhamnoides | en_US |
dc.subject | mitochondria | en_US |
dc.subject | radioprotection | en_US |
dc.subject.mesh | Antioxidants --pharmacology | |
dc.subject.mesh | Apoptosis --drug effects | |
dc.subject.mesh | Apoptosis --radiation effects | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Cell Survival --drug effects | |
dc.subject.mesh | Cell Survival --radiation effects | |
dc.subject.mesh | Free Radicals --metabolism | |
dc.subject.mesh | Gamma Rays --adverse effects | |
dc.subject.mesh | Hippophae | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Membrane Potential, Mitochondrial --drug effects | |
dc.subject.mesh | Membrane Potential, Mitochondrial --radiation effects | |
dc.subject.mesh | Plant Extracts --pharmacology | |
dc.subject.mesh | Plants, Medicinal | |
dc.subject.mesh | Radiation-Protective Agents --pharmacology | |
dc.title | Modulation of radiation-induced cytotoxicity in U 87 cells by RH-3 (a preparation of Hippophae rhamnoides). | en_US |
dc.type | Article | en_US |