Tissue Distribution Study On Apocynin Nitrone Derivative AN-1 After Intravenous Administration

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dc.contributor.authorLi, Huirouen_US
dc.contributor.authorLiang, Zhiyanen_US
dc.contributor.authorWang, Kaiyuen_US
dc.contributor.authorOuyang, Jiabien_US
dc.contributor.authoral., Mohui Yang eten_US
dc.date.accessioned2020-11-18T10:29:12Z
dc.date.available2020-11-18T10:29:12Z
dc.date.issued2019-06
dc.description.abstractBackground Apocynin, a main component extracted from the root of Picrorhiza kurroa Royle, was a well-knownNADPH oxidase inhibitor and reported to have effect on lung injury, liver injury, diabetes and asthma. AN-1, anitrone derivative of apocynin, was found to exhibit significant effect on treatment of acute lung injury.Aim In order to carry out further preclinical study, it is important to reveal in vivo disposition of AN-1. A simple andrapid high-performance liquid chromatography (HPLC) method was developed to disclose the tissue distributionbehavior of AN-1 in Sprague-Dawley (SD) rats.Methods A HPLC method was developed and validated to measure the concentration of AN-1 in tissue sampleswith carbamazepine as internal standard (IS). The mobile phase consisted of water and methanol (47:53, v/v), theflow rate was 1 mL/min, and an ultraviolet (UV) detector was used at wavelength of 279 nm. The tissue distributionstudy of AN-1 was performed in Sprague-Dawley (SD) rats after a single intravenous dose of 40 mg/kg.Results The developed HPLC-UV method was of good specificity, precision (< 4%), accuracy (90-97%) and recovery(88-104%) for analysis of AN-1 in tissue samples of rats. The linear range was established over a concentrationrange 0.2-50 µg/mL (r2 > 0.998) in tissues including heart, liver, spleen, lung, kidney and brain. After administration,AN-1 was rapidly distributed in tissues and reached peak concentration with time, which showed a high distributionen_US
dc.identifier.affiliationsCollege of Pharmacy, Jinan University, Guangzhou 510632, Chinaen_US
dc.identifier.affiliationsDispensary of Traditional Chinese Medicine, Hainan Provincial Hospital of Traditional Chinese Medicine, Haikou 570203, Chinaen_US
dc.identifier.affiliationsDongguan Institute of Jinan University, Dongguan 523808, Chinaen_US
dc.identifier.citationLi Huirou, Liang Zhiyan, Wang Kaiyu, Ouyang Jiabi, al. Mohui Yang et. Tissue Distribution Study On Apocynin Nitrone Derivative AN-1 After Intravenous Administration. Journal of Pharmaceutical and Biomedical Sciences. 2019 Jun; 9(6): 62-70en_US
dc.identifier.issn2230-7885
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215726
dc.languageenen_US
dc.publisherLawarence Press Pvt. Ltd.en_US
dc.relation.issuenumber6en_US
dc.relation.volume9en_US
dc.source.urihttps://lawarencepress.com/ojs/index.php/JPBMS/article/view/565/pdf_281en_US
dc.subjectAN-1en_US
dc.subjectApocyninen_US
dc.subjectnitrone derivationen_US
dc.subjecttissue distributionen_US
dc.titleTissue Distribution Study On Apocynin Nitrone Derivative AN-1 After Intravenous Administrationen_US
dc.typeJournal Articleen_US
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