Determination of malathion in blood and urine using thin-layer chromatography.

Simple item page
dc.contributor.authorJaiswal, A K
dc.contributor.authorKaur, Ashmeet
dc.contributor.authorMillo, T
dc.contributor.authorKumar, Adarsh
dc.contributor.authorGupta, M
dc.date.accessioned2011-10-04T05:42:48Z
dc.date.available2011-10-04T05:42:48Z
dc.date.issued2008-01
dc.description.abstractMalathion is a broad-spectrum Organophosphorus insecticide generally used to control a variety of insects. Routinely it is analysed by Gas Liquid Chromatography & High Performance Liquid Chromatography. Now an attempt has been made to develop a new method for analysis of Malathion in biological samples using Thin Layer Chromatography (TLC) technique, which is inexpensive, accurate and non-destructive. Malathion was extracted from blood and urine using solvent extraction methods and then identified on the TLC plates. For chromatographic separation, various binary and tertiary solvent systems were used to demonstrate in the laboratory. For detection on developed plates, palladium chloride reagent was used which successfully increased the sensitivity without dispensing with the simplicity of the method. For the analysis, a total of 20 solvent systems were chosen. Out of these 20 solvent systems, the three best solvent systems namely Cyclohexane: Chloroform (60:40), Hexane: Acetone (80:20) and Acetone: Hexane: Toluene (40:40:20) were chosen for statistical analysis which included the calculation of mean Rf value, value of standard deviation and coefficient of variance. The method developed is simple, inexpensive, accurate and non-destructive that allows for sensitive and reproducible analysis of Malathion.en_US
dc.identifier.citationJaiswal AK, Kaur Ashmeet, Millo T, Kumar Adarsh,Gupta M. Determination of malathion in blood and urine using thin-layer chromatography. Journal of Indian Academy of Forensic Medicine. 2008 Jan; 30(1): 15-21.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/134823
dc.language.isoenen_US
dc.source.urihttps://www.forensicindia.com/journals/jiafm/t08/i1/jalt08i1p15.pdfen_US
dc.subjectOrganophosphorus compounden_US
dc.subjectMalathionen_US
dc.subjectpalladium chlorideen_US
dc.subjecthRfen_US
dc.subjectTLC plateen_US
dc.subject.meshChromatography, Thin Layer --methods
dc.subject.meshHumans
dc.subject.meshMalathion --analysis
dc.subject.meshMalathion --blood
dc.subject.meshMalathion --urine
dc.subject.meshPalladium --diagnostic use
dc.titleDetermination of malathion in blood and urine using thin-layer chromatography.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
jiafm2008v30i1p15.pdf
Size:
43.77 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal of Indian Academy of Forensic Medicine