Enhancement of resistance to aphids by introducing the snowdrop lectin gene gna into maize plants.

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dc.contributor.authorWang, Zhaoyuen_US
dc.contributor.authorZhang, Keweien_US
dc.contributor.authorSun, Xiaofenen_US
dc.contributor.authorTang, Kexuanen_US
dc.contributor.authorZhang, Jurenen_US
dc.date.accessioned2005-12-03en_US
dc.date.accessioned2009-06-01T15:02:14Z
dc.date.available2005-12-03en_US
dc.date.available2009-06-01T15:02:14Z
dc.date.issued2005-12-03en_US
dc.description.abstractIn order to enhance the resistance to pests, transgenic maize (Zea mays L.) plants from elite inbred lines containing the gene encoding snowdrop lectin (Galanthus nivalis L. agglutinin; GNA) under control of a phloem-specific promoter were generated through the Agrobacterium tumefaciens-mediated method. The toxicity of GNA-expressing plants to aphids has also been studied. The independently derived plants were subjected to molecular analyses. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that the gna gene was integrated into maize genome and inherited to the following generations. The typical Mendelian patterns of inheritance occurred in most cases. The level of GNA expression at 0.13%-0.28% of total soluble protein was observed in different transgenic plants. The progeny of nine GNA-expressing independent transformants that were derived separately from the elite inbred lines DH4866, DH9942, and 8902, were selected for examination of resistance to aphids. These plants synthesized GNA at levels above 0.22% total soluble protein, and enhanced resistance to aphids was demonstrated by exposing the plants to corn leaf aphid (Rhopalosiphum maidis Fitch) under greenhouse conditions. The nymph production was significantly reduced by 46.9% on GNA-expressing plants. Field evaluation of the transgenic plants supported the results from the inoculation trial. After a series of artificial self-crosses, some homozygous transgenic maize lines expressing GNA were obtained. In the present study, we have obtained new insect-resistant maize material for further breeding work.en_US
dc.description.affiliationLife Science School, Shandong University, Jinan 250100, People's Republic of China.en_US
dc.identifier.citationWang Z, Zhang K, Sun X, Tang K, Zhang J. Enhancement of resistance to aphids by introducing the snowdrop lectin gene gna into maize plants. Journal of Biosciences. 2005 Dec; 30(5): 627-38en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111256
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAphids --growth & developmenten_US
dc.subject.meshBiological Assayen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshFertilityen_US
dc.subject.meshInsect Controlen_US
dc.subject.meshInsecticidesen_US
dc.subject.meshMannose-Binding Lectins --geneticsen_US
dc.subject.meshPest Control, Biologicalen_US
dc.subject.meshPlant Lectins --geneticsen_US
dc.subject.meshPlants, Genetically Modifieden_US
dc.subject.meshPlasmidsen_US
dc.subject.meshRhizobium radiobacter --geneticsen_US
dc.subject.meshSurvival Analysisen_US
dc.subject.meshTransformation, Geneticen_US
dc.subject.meshZea mays --geneticsen_US
dc.titleEnhancement of resistance to aphids by introducing the snowdrop lectin gene gna into maize plants.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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