Development of a LC-MS/MS Method for a New Ortho-aryl Chalcone Compound of OC26 and its Application to Bile

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dc.contributor.authorChen, Lexingen_US
dc.contributor.authorLiu, Jiangen_US
dc.contributor.authorGan, Xiaen_US
dc.contributor.authorJiang, Zhounanen_US
dc.contributor.authorXu, Junen_US
dc.contributor.authorCai, Shaohuien_US
dc.date.accessioned2020-11-18T10:29:12Z
dc.date.available2020-11-18T10:29:12Z
dc.date.issued2020-02
dc.description.abstractBackground OC26 and its pro-drug BOC26P, both ortho-aryl chalcone compounds, showed a well-definedantitumor activity in various cancer cells especially in drug-resistant tumor cell lines.Aim The purpose of this study was to investigate the bile excretion characteristics of OC26 after OC26 and BOC26Padministered in rats respectively.Method An ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method wasdeveloped and validated for OC26 in rat bile. Liquid-liquid extraction with ethyl acetate method was use to pretreatthe bile samples. After that, a gradient mobile phase at a flow rate 0.5mL/min of acetonitrile and 2mM CH3COONH4with 0.1% aqueous ammonia solution (v/v) and the positive ion alternate mode separated and quantified OC26.Bile samples were collected from rats after intravenous injection (i.v) 12.5mg/kg of OC26 and BOC26P, respectively.Results For method validation, the method showed high extraction recovery. The assay showed a good linearitywith correlation coefficient >0.99 at the concentration ranges of 20-2000ng/mL. All data were within the requiredlimits. The bile excretion results showed that the excretion amount of OC26 was gradually stabilized after 2h. Theaccumulative excretion percentage of OC26 after i.v 12.5mg/kg BOC26P was significantly higher than that of OC26after i.v 12.5mg/kg OC26. Significant gender differences were also observed in bile excretion of OC26.Conclusion This method was selective, sensitive and reliable and successfully applied to the bile excretion of OC26.This study provided theoretical basis for OC26 further research.en_US
dc.identifier.affiliationsCollege of Pharmacy, Jinan University, Guangzhou 510632, PR Chinaen_US
dc.identifier.affiliationsSichuan Greentech Biotechnology Co., Ltd, Meishan 620042, PR Chinaen_US
dc.identifier.affiliationsBoth Jun Xu and Shaohui Cai are corresponding authorsen_US
dc.identifier.citationChen Lexing, Liu Jiang, Gan Xia, Jiang Zhounan, Xu Jun, Cai Shaohui. Development of a LC-MS/MS Method for a New Ortho-aryl Chalcone Compound of OC26 and its Application to Bile. Journal of Pharmaceutical and Biomedical Sciences. 2020 Feb; 10(2): 10-18en_US
dc.identifier.issn2230-7885
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215732
dc.languageenen_US
dc.publisherLawarence Press Pvt. Ltd.en_US
dc.relation.issuenumber2en_US
dc.relation.volume10en_US
dc.source.urihttps://lawarencepress.com/ojs/index.php/JPBMS/article/view/596/pdf_286en_US
dc.subjectOC26en_US
dc.subjectBOC26Pen_US
dc.subjectLC-MS/MSen_US
dc.subjectBile excretionen_US
dc.subjectActive metaboliteen_US
dc.titleDevelopment of a LC-MS/MS Method for a New Ortho-aryl Chalcone Compound of OC26 and its Application to Bileen_US
dc.typeJournal Articleen_US
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