The inner workings of intracellular heterotypic and homotypic membrane fusion mechanisms

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dc.contributor.authorCruz, Mariel Delgadoen_US
dc.contributor.authorKim, Kyoungtaeen_US
dc.date.accessioned2020-11-18T10:14:31Z
dc.date.available2020-11-18T10:14:31Z
dc.date.issued2019-09
dc.description.abstractThe glycolytic enzyme enolase of Staphylococcus aureus is a highly conserved enzyme which binds to human plasminogenthereby aiding the infection process. The cloning, over expression and purification of S. aureus enolase as well as the effectof various metals upon the catalytic activity and structural stability of the enzyme have been reported. The recombinantenzyme (rSaeno) has been purified to homogeneity in abundant amounts (60 mg/L of culture) and the kinetic parameters(Km = 0.23 ± 0.013 9 10-3 M; Vmax = 90.98 ± 0.00052 U/mg) and the optimum pH were calculated. This communication further reports that increasing concentrations of Na? ions inhibit the enzyme while increasing concentrations of K?ions were stimulatory. In case of divalent cations, it was found that Mg2? stimulates the activity of rSaeno while the rest ofthe divalent cations (Zn2?, Mn2?, Fe2?, Cu2?, Ni2? and Ca2?) lead to a dose-dependent loss in the activity with a total lossof activity in the presence of Hg2? and Cr2?. The circular dichroism data indicate that other than Hg2?, Ni2? and to acertain extent Cu2?, none of the other ions destabilized rSaeno. The inhibitory roles of fluorides, as well as neurotoxiccompounds upon the catalytic activity of rSaeno, have also been studied. Conformational changes in rSaeno (induced byions) were studied using partial trypsin digestion.en_US
dc.identifier.affiliationsMissouri State University, 901 S. National Ave, Springfield, MO 65897, USAen_US
dc.identifier.affiliationsMissouri State University, 901 S. National Ave, Springfield, MO 65897, USAen_US
dc.identifier.citationCruz Mariel Delgado, Kim Kyoungtae. The inner workings of intracellular heterotypic and homotypic membrane fusion mechanisms. Journal of Biosciences. 2019 Sep; 44(4): 1-14en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214436
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.issuenumber4en_US
dc.relation.volume44en_US
dc.source.urihttps://dx.doi.org//10.1007/s12038-019-9913-3en_US
dc.subjectGolgien_US
dc.subjectRabsen_US
dc.subjectSNAREsen_US
dc.subjecttethersen_US
dc.subjectvacuoleen_US
dc.titleThe inner workings of intracellular heterotypic and homotypic membrane fusion mechanismsen_US
dc.typeJournal Articleen_US
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