Single-nucleotide polymorphisms in CLEC7A, CD209 and TLR4 gene and their association with susceptibility to paratuberculosis in Indian cattle

Simple item page
dc.contributor.authorGopi, B.en_US
dc.contributor.authorSINGH, Ran Viren_US
dc.contributor.authorKumar, Satishen_US
dc.contributor.authorKumar, Sushilen_US
dc.contributor.authorChauhan, Anujen_US
dc.contributor.authorKumar, Amiten_US
dc.contributor.authorSingh, Shoor Viren_US
dc.date.accessioned2020-11-18T10:21:10Z
dc.date.available2020-11-18T10:21:10Z
dc.date.issued2020-02
dc.description.abstractThe aim of this study was to identify the single-nucleotide polymorphisms (SNPs) in bovine candidate genes CLEC7A, CD209 and TLR4, and explore the association between these SNPs with the occurrence of bovine paratuberculosis (PTB) disease. For this purpose, 549 animals were screened by a panel of four diagnostic tests, namely Johnin PPD test, ELISA test, faecal microscopy and IS900 blood PCR against Mycobacterium avium ssp. paratuberculosis (MAP) to develop case–control populations. SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Genotypic–phenotypic associations were assessed by the PROCLOGISTIC procedure of SAS 9.3. Of the seven SNPs; rs110353594 in CLEC7A gene and rs8193046 in TLR4 gene were found to be associated with PTB. For SNP rs110353594, odds of CC and CT genotypes vs TT genotype was 1.543 (0.420–5.667; 95% CI) and 0.284 (0.104–0.774; 95% CI), respectively which means that CT genotype was more resistant than TT and CC genotypes against bovine PTB. For SNP rs8193046, odds of AA and AG genotypes versus GG genotype was 0.947 (0.296–3.034; 95% CI) and 3.947 (1.555–10.022; 95% CI), respectively, i.e. probability for getting an infection in animals with AG genotype was 3.94 times more as compared to GG genotype. Hence, a selection programme favouring CT genotype for rs110353594 and against AG genotype for rs8193046 may be beneficial for conferring resistance against bovine PTBen_US
dc.identifier.affiliationsDivision of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, Indiaen_US
dc.identifier.affiliationsDivision of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, Indiaen_US
dc.identifier.affiliationsICAR-National Research Centre on Pig, Rani, Guwahati 781 131, Indiaen_US
dc.identifier.affiliationsDivision of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, Indiaen_US
dc.identifier.affiliationsDivision of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, Indiaen_US
dc.identifier.affiliationsDivision of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, Indiaen_US
dc.identifier.affiliationsICAR-Central Institute for Research on Goat, Makhdoom 281 122, Indiaen_US
dc.identifier.citationGopi B., Singh Ran Vir, Kumar Satish, Kumar Sushil, Chauhan Anuj, Kumar Amit, Singh Shoor Vir. Single-nucleotide polymorphisms in CLEC7A, CD209 and TLR4 gene and their association with susceptibility to paratuberculosis in Indian cattle. Journal of Genetics. 2020 Feb; 99: 1-10en_US
dc.identifier.issn0022-1333
dc.identifier.issn0973-7731
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215549
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.volume99en_US
dc.source.urihttps://doi.org/10.1007/s12041-019-1172-4en_US
dc.subjectimmune responseen_US
dc.subjectparatuberculosisen_US
dc.subjectresistanceen_US
dc.subjectsingle-nucleotide polymorphismen_US
dc.subjectTLR4 gene.en_US
dc.titleSingle-nucleotide polymorphisms in CLEC7A, CD209 and TLR4 gene and their association with susceptibility to paratuberculosis in Indian cattleen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
jmsh2019v99prrr1.pdf
Size:
332.13 KB
Format:
Adobe Portable Document Format
Download
Collections
Journal of Genetics