Human oocyte chromosome analysis: complicated cases and major pitfalls.

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dc.contributor.authorRosenbusch, Bernden_US
dc.contributor.authorSchneider, Michaelen_US
dc.contributor.authorMichelmann, Hans Wilhelmen_US
dc.date.accessioned2008-08-09en_US
dc.date.accessioned2009-06-02T07:07:23Z
dc.date.available2008-08-09en_US
dc.date.available2009-06-02T07:07:23Z
dc.date.issued2008-08-09en_US
dc.description.abstractHuman oocytes that remained unfertilized in programmes of assisted reproduction have been analysed cytogenetically for more than 20 years to assess the incidence of aneuploidy in female gametes. However, the results obtained so far are not indisputable as a consequence of difficulties in evaluating oocyte chromosome preparations. Because of the lack of guidelines, we decided to summarize for the first time, the possible pitfalls in human oocyte chromosome analysis. Therefore, we screened the material from our previous studies and compiled representative, complicated cases with recommendations for their cytogenetic classification. We point out that maturity and size of the oocyte are important parameters and that fixation artefacts, as well as the particular structure of oocyte chromosomes, may predispose one to misinterpretations. Moreover, phenomena related to oocyte activation and fertilization are illustrated and explained. This compilation may help to avoid major problems in future studies and contribute to a more precise, and uniform assessment of human oocyte chromosomes.en_US
dc.description.affiliationDepartment of Gynecology and Obstetrics, University of Ulm, Prittwitzstrasse 43, 89075 Ulm, Germany. bernd.rosenbusch@uniklinik-ulm.deen_US
dc.identifier.citationRosenbusch B, Schneider M, Michelmann HW. Human oocyte chromosome analysis: complicated cases and major pitfalls. Journal of Genetics. 2008 Aug; 87(2): 147-53en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/114229
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jgenet/index.htmlen_US
dc.subject.meshChromatids --metabolismen_US
dc.subject.meshChromatin --metabolismen_US
dc.subject.meshChromosome Aberrationsen_US
dc.subject.meshChromosomes, Human --metabolismen_US
dc.subject.meshDiploidyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFertilizationen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMitosisen_US
dc.subject.meshOocytes --cytologyen_US
dc.subject.meshPolyploidyen_US
dc.subject.meshSperm Injections, Intracytoplasmicen_US
dc.subject.meshSpermatozoa --cytologyen_US
dc.subject.meshTissue Fixationen_US
dc.titleHuman oocyte chromosome analysis: complicated cases and major pitfalls.en_US
dc.typeJournal Articleen_US
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