Association between KRAS, NRAS, and BRAF mutations and tumor localization in colorectal cancer patients in BSMMU

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dc.contributor.authorSiddiquee, MAen_US
dc.contributor.authorRahman, SAMen_US
dc.contributor.authorIslam, MSen_US
dc.contributor.authorOvi, MRAen_US
dc.contributor.authorJalal, MTen_US
dc.contributor.authorHaque, AAen_US
dc.contributor.authorSaha, KKen_US
dc.contributor.authorHossain, Ten_US
dc.contributor.authorAli, MSen_US
dc.contributor.authorAkter, J.en_US
dc.date.accessioned2025-05-12T09:33:02Z
dc.date.available2025-05-12T09:33:02Z
dc.date.issued2024-12
dc.description.abstractBackground: Colorectal cancer (CRC) is a common malignancy with significant genetic heterogeneity. Mutations in proto-oncogenes such as KRAS, NRAS, and BRAF play a pivotal role in CRC development, impacting prognosis and treatment. This study aims to correlate mutations in these genes with tumor localization in both primary and metastatic CRC in the Bangabandhu Sheikh Mujib medical university (BSMMU) cohort. Methods: This prospective cross-sectional study was conducted between July 2023 and June 2024 at BSMMU. A total of 30 CRC patients, confirmed via histopathology, were included. Purposive sampling was used to select patients. Tumor tissue samples were collected and analyzed for KRAS, NRAS, and BRAF mutations using DNA isolation, PCR amplification, and sequencing techniques. Results: Among the 30 patients, the majority were male (66.7%) with a mean age of 50.4 years. KRAS mutations were found in 5 patients (16.7%), while no mutations in NRAS or BRAF were detected. Rectal cancer was the most frequent tumor location (36.7%), followed by hepatic and splenic flexure (16.7% each). No significant correlation was observed between KRAS mutations and tumor localization?. Conclusions: There was no statistically significant correlation between KRAS, NRAS, and BRAF mutations and tumor localization in the BSMMU CRC patient cohort. The study highlights the need for larger sample sizes to better understand the genetic landscape of CRC in Bangladesh?. Small sample size may limit the ability to detect significant associations. Further large-scale studies could offer more conclusive insights.en_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Colorectal Surgery, BSMMU, Dhaka, Bangladeshen_US
dc.identifier.citationSiddiquee MA, Rahman SAM, Islam MS, Ovi MRA, Jalal MT, Haque AA, Saha KK, Hossain T, Ali MS, Akter J.. Association between KRAS, NRAS, and BRAF mutations and tumor localization in colorectal cancer patients in BSMMU . International Surgery Journal. 2024 Dec; 11(12): 1998-2001en_US
dc.identifier.issn2349-3305
dc.identifier.issn2349-2902
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/247259
dc.languageenen_US
dc.publisherMedip Academyen_US
dc.relation.issuenumber12en_US
dc.relation.volume11en_US
dc.source.urihttps://doi.org/10.18203/2349-2902.isj20243539en_US
dc.subjectColorectal canceren_US
dc.subjectKRASen_US
dc.subjectNRASen_US
dc.subjectBRAFen_US
dc.subjectDNA isolationen_US
dc.subjectPCR amplificationen_US
dc.titleAssociation between KRAS, NRAS, and BRAF mutations and tumor localization in colorectal cancer patients in BSMMUen_US
dc.typeJournal Articleen_US
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